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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse spleen tissue using TFCP2 Polyclonal antibody (15203-1-AP)

    WB analysis of mouse spleen using 15203-1-AP (same clone as 15203-1-PBS)

    mouse spleen tissue were subjected to SDS PAGE followed by western blot with 15203-1-AP (TFCP2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15203-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat brain tissue using TFCP2 Polyclonal antibody (15203-1-AP)

    WB analysis of rat brain using 15203-1-AP (same clone as 15203-1-PBS)

    rat brain tissue were subjected to SDS PAGE followed by western blot with 15203-1-AP (TFCP2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15203-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat spleen tissue using TFCP2 Polyclonal antibody (15203-1-AP)

    WB analysis of rat spleen using 15203-1-AP (same clone as 15203-1-PBS)

    rat spleen tissue were subjected to SDS PAGE followed by western blot with 15203-1-AP (TFCP2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15203-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse kidney tissue using TFCP2 Polyclonal antibody (15203-1-AP)

    WB analysis of mouse kidney using 15203-1-AP (same clone as 15203-1-PBS)

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 15203-1-AP (TFCP2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15203-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of K-562 cells using TFCP2 Polyclonal antibody (15203-1-AP)

    WB analysis of K-562 using 15203-1-AP (same clone as 15203-1-PBS)

    K-562 cells were subjected to SDS PAGE followed by western blot with 15203-1-AP (TFCP2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15203-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of mouse spleen tissue using TFCP2 Polyclonal antibody (15203-1-AP)

    IP experiment of mouse spleen using 15203-1-AP (same clone as 15203-1-PBS)

    IP result of anti-TFCP2 (IP:15203-1-AP, 4ug; Detection:15203-1-AP 1:500) with mouse spleen tissue lysate 4000 ug. This data was developed using the same antibody clone with 15203-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

TFCP2 Polyclonal antibody, PBS Only

TFCP2 Polyclonal Antibody for WB, IP, Indirect ELISA
Cat No. 15203-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

15203-1-PBS targets TFCP2 in WB, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag7353

Product name: Recombinant human TFCP2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-348 aa of BC003634

Sequence: MAWALKLPLADEVIESGLVQDFDASLSGIGQELGAGAYSMSDVLALPIFKQEESSLPPDNENKILPFQYVLCAATSPAVKLHDETLTYLNQGQSYEIRMLDNRKLGELPEINGKLVKSIFRVVFHDRRLQYTEHQQLEGWRWNRPGDRILDIDIPMSVGIIDPRANPTQLNTVEFLWDPAKRTSVFIQVHCISTEFTMRKHGGEKGVPFRVQIDTFKENENGEYTEHLHSASCQIKVFKPKGADRKQKTDREKMEKRTPHEKEKYQPSYETTILTECSPWPEITYVNNSPSPGFNSSHSSFSLGEGNGSPNHQPEPPPPVTDNLLPTTTPQEAQQWLHRNRFSTFTRL

相同性解析による交差性が予測される生物種
Full Name transcription factor CP2
Calculated molecular weight 57 kDa
Observed molecular weight 66 kDa
GenBank accession numberBC003634
Gene Symbol TFCP2
Gene ID (NCBI) 7024
RRIDAB_2199582
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ12800
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

TFCP2, also named as SAA3 enhancer factor and transcription factor LSF, is a 502 amino acid protein, which belongs to the grh/CP2 family. TFCP2 is expressed in brain, ovary, kidney, thymus, spleen, liver, adrenal, heart and lung. TFCP2 binds a variety of cellular and viral promoters including fibrinogen, alpha-globin, SV40 and HIV-1 promoters. The calculated molecular weight of TFCP2 is 57 kDa Similar levels of TFCP2 migrating at 64-66 kDa were evident in all three samples. (PMID: 7828600)

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