• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Use Able AI chat for product recommendations

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using TFB1M Recombinant monoclonal antibody (84957-4-RR)

    WB analysis using 84957-4-RR (same clone as 84957-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84957-4-RR (TFB1M antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84957-4-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HepG2 cells using TFB1M Recombinant monoclonal antibody (84957-4-RR)

    IP experiment of HepG2 using 84957-4-RR (same clone as 84957-4-PBS)

    IP result of anti-TFB1M (IP:84957-4-RR, 4ug; Detection:84957-4-RR 1:1500) with HepG2 cells lysate 1600 ug. This data was developed using the same antibody clone with 84957-4-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using TFB1M Recombinant monoclonal antibody (84957-4-RR)

    IHC staining of human colon cancer using 84957-4-RR (same clone as 84957-4-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 84957-4-RR (TFB1M antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84957-4-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using TFB1M Recombinant monoclonal antibody (84957-4-RR)

    IHC staining of human colon cancer using 84957-4-RR (same clone as 84957-4-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 84957-4-RR (TFB1M antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84957-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84957-4-RR

    Affinity and Kinetic Characterization of 84957-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84957-4-RR against Human TFB1M were performed. The affinity constant is 2.99 nM.

TFB1M Recombinant monoclonal antibody, PBS Only

TFB1M Uni-rAb® Recombinant Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 84957-4-PBS
Clone No.242524A10

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IP, Indirect ELISA

Synonyms

242524A10, CGI 75, CGI75, CGI-75, EC:2.1.1.-

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

84957-4-PBS targets TFB1M in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag9785

Product name: Recombinant human TFB1M protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-346 aa of BC017788

Sequence: MAASGKLSTCRLPPLPTIREIIKLLRLQAAKQLSQNFLLDLRLTDKIVRKAGNLTNAYVYEVGPGPGGITRSILNADVAELLVVEKDTRFIPGLQMLSDAAPGKLRIVHGDVLTFKVEKAFSESLKRPWEDDPPNVHIIGNLPFSVSTPLIIKWLENISCRDGPFVYGRTQMTLTFQKEVAERLAANTGSKQRSRLSVMAQYLCNVRHIFTIPGQAFVPKPEVDVGVVHFTPLIQPKIEQPFKLVEKVVQNVFQFRRKYCHRGLRMLFPEAQRLESTGRLLELADIDPTLRPRQLSISHFKSLCDVYRKMCDEDPQLFAYNFREELKRRKSKNEEKEEDDAENYRL

相同性解析による交差性が予測される生物種
Full Name transcription factor B1, mitochondrial
Calculated molecular weight 346 aa, 40 kDa
Observed molecular weight40 kDa
GenBank accession numberBC017788
Gene Symbol TFB1M
Gene ID (NCBI) 51106
RRIDAB_3743884
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purfication
UNIPROT IDQ8WVM0
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Mitochondrial transcription factor B1 (TFB1M) is a dimethyltransferase that maintains mitochondrial homeostasis by catalyzing dimethylation of two adjacent adenines located in helix45 (h45) of 12S rRNA. Mitochondrial transcription specificity factors (TFB1M and TFB2M), which markedly enhance mtDNA transcription in the presence of Tfam and mitochondrial RNA polymerase, have been identified in mammalian cells. The expression of human TFB1M and TFB2M promoters is governed by nuclear respiratory factors (NRF-1 and NRF-2), key transcription factors implicated in mitochondrial biogenesis. (PMID: 31251801, PMID: 15684387)

||
New chat

Able™

正在加载，请稍候...