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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TFAP4 Recombinant antibody (83196-4-RR)

    WB analysis using 83196-4-RR (same clone as 83196-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83196-4-RR (TFAP4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83196-4-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using TFAP4 Recombinant antibody (83196-4-RR)

    WB analysis of HeLa using 83196-4-RR (same clone as 83196-4-PBS)

    WB result of TFAP4 antibody (83196-4-RR; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TFAP4 transfected HeLa cells. This data was developed using the same antibody clone with 83196-4-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using TFAP4 Recombinant antibody (83196-4-RR)

    IF Staining of HeLa using 83196-4-RR (same clone as 83196-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using TFAP4 antibody (83196-4-RR, Clone: 240031D5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83196-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83196-4-RR

    Affinity and Kinetic Characterization of 83196-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83196-4-RR against Human TFAP4 were performed. The affinity constant is below 1 pM.

  • Featured Product
  • KD/KO Validated

TFAP4 Recombinant monoclonal antibody, PBS Only

TFAP4 Uni-rAb® Recombinant Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 83196-4-PBS
Clone No.240031D5

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

240031D5, Activating enhancer-binding protein 4, AP 4, bHLHc41, Class C basic helix-loop-helix protein 41

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83196-4-PBS targets TFAP4 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag2633

Product name: Recombinant human TFAP4 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-338 aa of BC010576

Sequence: MEYFMVPTQKVPSLQHFRKTEKEVIGGLCSLANIPLTPETQRDQERRIRREIANSNERRRMQSINAGFQSLKTLIPHTDGEKLSKAAILQQTAEYIFSLEQEKTRLLQQNTQLKRFIQELSGSSPKRRRAEDKDEGIGSPDIWEDEKAEDLRREMIELRQQLDKERSVRMMLEEQVRSLEAHMYPEKLKVIAQQVQLQQQQEQVRLLHQEKLEREQQQLRTQLLPPPAPTHHPTVIVPAPPPPPSHHINVVTMGPSSVINSVSTSRQNLDTIVQAIQHIEGTQEKQELEEEQRRAVIVKPVRSCPEAPTSDTASDSEASDSDAMDQSREEPSGDGELP

相同性解析による交差性が予測される生物種
Full Name transcription factor AP-4 (activating enhancer binding protein 4)
Calculated molecular weight 338 aa, 39 kDa
Observed molecular weight39-42 kDa
GenBank accession numberBC010576
Gene Symbol TFAP4
Gene ID (NCBI) 7023
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purfication
UNIPROT IDQ01664
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.
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