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Species Reactivity
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Conjugate
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

TFAP4 Polyclonal antibody

TFAP4 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Cat no : 12017-1-AP

Synonyms

AP 4, bHLHc41, TFAP4, Transcription factor AP 4

Validation Data Gallery

Applications:
  • WB analysis using 12017-1-AP

    WB analysis using 12017-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12017-1-AP (TFAP4 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • WB analysis using 12017-1-AP

    WB analysis using 12017-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12017-1-AP (TFAP4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IP experiment of HeLa using 12017-1-AP

    IP experiment of HeLa using 12017-1-AP

    IP result of anti-TFAP4 (IP:12017-1-AP, 4ug; Detection:12017-1-AP 1:1000) with HeLa cells lysate 1360 ug.

Tested Applications

Positive WB detected inA549 cells, HeLa cells, Jurkat cells, NCI-H1299 cells
Positive IP detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 3 publications below

Product Information

12017-1-AP targets TFAP4 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TFAP4 fusion protein Ag2633 相同性解析による交差性が予測される生物種
Full Name transcription factor AP-4 (activating enhancer binding protein 4)
Calculated molecular weight 338 aa, 39 kDa
Observed molecular weight 39-42 kDa
GenBank accession numberBC010576
Gene symbol TFAP4
Gene ID (NCBI) 7023
RRIDAB_2199571
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for TFAP4 antibody 12017-1-APDownload protocol
IP protocol for TFAP4 antibody 12017-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Agric Food Chem

Cucurbitacin E Chemosensitizes Colorectal Cancer Cells via Mitigating TFAP4/Wnt/β-Catenin Signaling.

Authors - Peng Yang
View Article
humanWB

J Agric Food Chem

Cucurbitacin E Triggers Cellular Senescence in Colon Cancer Cells via Regulating the miR-371b-5p/TFAP4 Signaling Pathway.

Authors - Peng Yang
View Article
humanWB

Invest Ophthalmol Vis Sci

A Novel Small Peptide H-KI20 Inhibits Retinal Neovascularization Through the JNK/ATF2 Signaling Pathway.

Authors - Ruonan Wang
View Article