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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TFAM Recombinant monoclonal antibody (82745-5-RR)

    WB analysis using 82745-5-RR (same clone as 82745-5-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 82745-5-RR (TFAM antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82745-5-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using TFAM Recombinant monoclonal antibody (82745-5-RR)

    WB analysis using 82745-5-RR (same clone as 82745-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82745-5-RR (TFAM antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82745-5-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using TFAM Recombinant monoclonal antibody (82745-5-RR)

    WB analysis using 82745-5-RR (same clone as 82745-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82745-5-RR (TFAM antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82745-5-PBS in a different storage buffer formulation.

TFAM Recombinant monoclonal antibody, PBS Only

TFAM Uni-rAb® Recombinant Antibody for WB, Indirect ELISA
Cat No. 82745-5-PBS
Clone No.250652B11

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, Indirect ELISA

Synonyms

MtTF1, mtTFA, TCF6, TCF-6, Transcription factor 6-like 2

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

82745-5-PBS targets TFAM in WB, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

Peptide

相同性解析による交差性が予測される生物種
Full Name transcription factor A, mitochondrial
Calculated molecular weight 246 aa, 29 kDa
Observed molecular weight 25 kDa
GenBank accession numberBC126366
Gene Symbol TFAM
Gene ID (NCBI) 7019
RRIDAB_3743421
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDQ00059
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

TFAM, also named as TCF6L2, MtTF1, TCF6, TCF6L1, TCF6L3, and mtTFA, is a mitochondrial transcription factor that is a key activator of mitochondrial transcription as well as a participant in mitochondrial genome replication. It is involved in mitochondrial transcription regulation. TFAM is required for accurate and efficient promoter recognition by the mitochondrial RNA polymerase. It activates transcription by binding immediately upstream of transcriptional start sites. TFAM is able to unwind and bend DNA.

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