Validation Data Gallery
Tested Applications
| Positive WB detected in | MCF-7 cells, SKOV-3 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
27150-1-AP targets TET3 in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag25877 Product name: Recombinant human TET3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 316-360 aa of BC022243 Sequence: KKIQKEKLSTPEKIKQEALELAGITSDPGLSLKGGLSQQGLKPSL 相同性解析による交差性が予測される生物種 |
| Full Name | tet oncogene family member 3 |
| Calculated molecular weight | 1660 aa, 179 kDa |
| Observed molecular weight | 180 kDa |
| GenBank accession number | BC022243 |
| Gene Symbol | TET3 |
| Gene ID (NCBI) | 200424 |
| RRID | AB_3669585 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O43151 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Ten-eleven translocation protein 3 (TET3) belongs to the family of ten-eleven translocations (TETs) which induce DNA demethylation and gene regulation at the epigenetic level by converting 5-methylcytosine (5mC) to 5-hydroxymethylcytosine (5hmC) (PMID: 31656201). In ovarian cancer, TET3 is reported as an oncogene or tumor suppressor during tumorigenesis and overexpressed in ovarian cancer tissues (PMID: 27141829).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for TET3 antibody 27150-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |