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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

TENC1 Polyclonal antibody

TENC1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 28153-1-AP

Synonyms

C1 TEN, C1TEN, DKFZp686D13244, FLJ16320, KIAA1075, TENC1, Tensin 2, TNS2

Validation Data Gallery

Applications:
  • WB analysis using 28153-1-AP

    WB analysis using 28153-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28153-1-AP (TENC1 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver cancer using 28153-1-AP

    IHC staining of human liver cancer using 28153-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 28153-1-AP (TENC1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human liver cancer using 28153-1-AP

    IHC staining of human liver cancer using 28153-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 28153-1-AP (TENC1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inHeLa cells, mouse heart tissue, rat heart tissue
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

28153-1-AP targets TENC1 in WB, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TENC1 fusion protein Ag27731 相同性解析による交差性が予測される生物種
Full Name tensin like C1 domain containing phosphatase (tensin 2)
Calculated molecular weight 1419 aa, 154 kDa
Observed molecular weight140-153 kDa
GenBank accession numberBC142668
Gene symbol TENC1
Gene ID (NCBI) 23371
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for TENC1 antibody 28153-1-APDownload protocol
IHC protocol for TENC1 antibody 28153-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols