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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TEAD3 Polyclonal antibody (13120-1-AP)

    WB analysis using 13120-1-AP (same clone as 13120-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 13120-1-AP (TEAD3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13120-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using TEAD3 Polyclonal antibody (13120-1-AP)

    WB analysis using 13120-1-AP (same clone as 13120-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 13120-1-AP (TEAD3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13120-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat heart tissue using TEAD3 Polyclonal antibody (13120-1-AP)

    IHC staining of rat heart using 13120-1-AP (same clone as 13120-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 13120-1-AP (TEAD3 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13120-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse intestine using TEAD3 Polyclonal antibody (13120-1-AP)

    IHC staining of mouse intestine using 13120-1-AP (same clone as 13120-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse intestine tissue slide using 13120-1-AP (TEAD3 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13120-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using TEAD3 Polyclonal antibody (13120-1-AP)

    IF Staining of MCF-7 using 13120-1-AP (same clone as 13120-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using 13120-1-AP (TEAD3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 13120-1-PBS in a different storage buffer formulation.

TEAD3 Polyclonal antibody, PBS Only

TEAD3 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 13120-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

DTEF 1, DTEF-1, ETFR 1, TEA domain family member 3, TEAD 3

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

13120-1-PBS targets TEAD3 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag3875

Product name: Recombinant human TEAD3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-324 aa of BC027877

Sequence: MNLDQVSKDKALQSMASMSSAQIVSASVLQNKFSPPSPLPQAVFSTSSRFWSSPPLLGQQPGPSQDIKPFAQPAYPIQPPLPPTLSSYEPLAPLPSAAASVPVWQDRTIASSRLRLLEYSAFMEVQRDPDTYSKHLFVHIGQTNPAFSDPPLEAVDVRQIYDKFPEKKGGLKELYEKGPPNAFFLVKFWADLNSTIQEGPGAFYGVSSQYSSADSMTISVSTKVCSFGKQVVEKVETEYARLENGRFVYRIHRSPMCEYMINFIHKLKHLPEKYMMNSVLENFTILQVVTSRDSQETLLVIAFVFEVSTSEHGAQHHVYKLVKD

相同性解析による交差性が予測される生物種
Full Name TEA domain family member 3
Calculated molecular weight 435 aa, 49 kDa
Observed molecular weight50 kDa
GenBank accession numberBC027877
Gene Symbol TEAD3
Gene ID (NCBI) 7005
RRIDAB_2203068
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ99594
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

TEAD3, others named ranscriptional enhancer factor 5(TEF5), a member of family of transcription factors that plays a key role in the Hippo signaling pathway, suggesting involve in organ and tumor suppression by restricting proliferation and promoting. TEAD3 contains the TEA/ATTS DNA-binding domain. Human TEAD3 has a strong mRNA expression in placenta and choriocarcinoma cells(JEG-3), but low in Hela, HepG2 and MCF-7. This is a rabbit polyclonal anti-TEAD3 antibody raised against part of C-terminal chain of human TEAD3.

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