Validation Data Gallery
Tested Applications
| Positive WB detected in | BGC-823 cells, A375 cells, C2C12 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 12 publications below |
| IF | See 1 publications below |
Product Information
13283-1-AP targets TEAD1 in WB, IF, ELISA applications and shows reactivity with mouse, human samples.
| Tested Reactivity | mouse, human |
| Cited Reactivity | human, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag4082 Product name: Recombinant human TEAD1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-60 aa of BC026959 Sequence: MLTPSIVYECATRNFQRAPFTIWHFQRMFQPSKGQLFKVLVGFYPNLVEIGKADRGKEDE 相同性解析による交差性が予測される生物種 |
| Full Name | TEA domain family member 1 (SV40 transcriptional enhancer factor) |
| Calculated molecular weight | 426 aa, 47, 9 kDa |
| Observed molecular weight | 52 kDa |
| GenBank accession number | BC026959 |
| Gene Symbol | TEAD1 |
| Gene ID (NCBI) | 7003 |
| RRID | AB_2287290 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P28347 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
TEAD1 belongs to a protein family that share the TEA/ATTS domain. It acts as a transcriptional factor by binding to the GT-IIC and Sph(I 1 II) enhansons in the simian virus 40 (SV40) enhancer. Also it can modulate SV40 late transcription together with large T antigen. The transcriptional activity of TEAD1 required several regions of the proteins , a TBP-associated factors and a limiting transcriptional intermediary factors. TEAD1 also preforms an essential role in the Hippo signaling pathway. TEAD1 regulates gene expression of YAP1 and WWTR1/TAZ, thus mediating cell proliferation, migration and epithelial mesenchymal transition induction. TEAD is preferentially expressed in skeletal muscle. Lower levels in pancreas, placenta, and heart.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for TEAD1 antibody 13283-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cancer Lett EGFR/c-myc axis regulates TGFβ/Hippo/Notch pathway via epigenetic silencing miR-524 in gliomas. | ||
Cancers (Basel) WISP2/CCN5 Suppresses Vasculogenic Mimicry through Inhibition of YAP/TAZ Signaling in Breast Cancer Cells. | ||
Int J Mol Sci Role of Clostridium perfringens Enterotoxin on YAP Activation in Colonic Sessile Serrated Adenoma/ Polyps with Dysplasia. | ||
Dig Dis Sci TEA Domain Transcription Factor 1 Inhibits Ferroptosis and Sorafenib Sensitivity of Hepatocellular Carcinoma Cells | ||
Biomed Pharmacother YAP1-TEAD1-Glut1 axis dictates the oncogenic phenotypes of breast cancer cells by modulating glycolysis. | ||
Oncol Rep YAP is overexpressed in clear cell renal cell carcinoma and its knockdown reduces cell proliferation and induces cell cycle arrest and apoptosis. |
