Validation Data Gallery
Tested Applications
| Positive WB detected in | MCF7 cells, HeLa cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human ovary cancer tissue, human intrahepatic cholangiocarcinoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 3 publications below |
| IF | See 1 publications below |
Product Information
10641-1-AP targets TDP1 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag0964 Product name: Recombinant human TDP1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-298 aa of BC006083 Sequence: MSQEGDYGRWTISSSDESEEEKPKPDKPSTSSLLCARQGAANEPRYTCSEAQKAAHKRKISPVKFSNTDSVLPPKRQKSGSQEDLGWCLSSSDDELQPEMPQKQAEKVVIKKEKDISAPNDGTAQRTENHGAPACHRLKEEEDEYETSGEGQDIWDMLDKGNPFQFYLTRVSGVKPKYNSGALHIKDILSPLFGTLVSSAQFNYCFDVDWLVKQYPPEFRKKPILLVHGDKREAKAHLHAQAKPYENISLCQAKLDIAFGTHHTKMMLLLYEEGLRVVIHTSNLIHADWHQKTQGTHL 相同性解析による交差性が予測される生物種 |
| Full Name | tyrosyl-DNA phosphodiesterase 1 |
| Calculated molecular weight | 34 kDa, 68 kDa |
| Observed molecular weight | 68 kDa |
| GenBank accession number | BC006083 |
| Gene Symbol | TDP1 |
| Gene ID (NCBI) | 55775 |
| RRID | AB_2240520 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9NUW8 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Tyrosyl DNA phosphodiesterase 1 (TDP1) is an enzyme capable of hydrolyzing phosphodiester bonds between tyrosine and the 3-phosphate of DNA, which are typically generated in a transient manner by DNA topoisomerase I (topo I) and it appears to be more mobile and diffusible than topo I, and it has a different distribution in the nucleus (PMID:15494395). TDP1 is involed in protecting cells against oxidative DNA damage, and with the impaired ability of TDP1-deficient cells to remove 3-phosphoglycolate (PMID:21041670). Defects in TDP1 are the cause of spinocerebellar ataxia autosomal recessive with axonal neuropathy (SCAN1)(PMID:17948061).
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for TDP1 antibody 10641-1-AP | Download protocol |
| IP protocol for TDP1 antibody 10641-1-AP | Download protocol |
| WB protocol for TDP1 antibody 10641-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Biochem Pharmacol Role of tyrosyl-DNA phosphodiesterase 1 and inter-players in regulation of tumor cell sensitivity to topoisomerase I inhibition. | ||
DNA Repair (Amst) TDP1 is required for efficient non-homologous end joining in human cells.
| ||
Int J Mol Sci Combined PARP and Dual Topoisomerase Inhibition Potentiates Genome Instability and Cell Death in Ovarian Cancer | ||
Anal Chem Spike-In Proteome Enhances Data-Independent Acquisition for Thermal Proteome Profiling |
