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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TDP-43 (C-terminal) Polyclonal antibody (12892-1-AP)

    WB analysis using 12892-1-AP (same clone as 12892-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 12892-1-AP (TDP-43 (C-terminal) antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12892-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of A549 cells using TDP-43 (C-terminal) Polyclonal antibody (12892-1-AP)

    WB analysis of A549 cells using 12892-1-AP (same clone as 12892-1-PBS)

    A549 cells (shcontrol and shRNA of TDP43) were subjected to SDS PAGE followed by western blot with 12892-1-AP (TDP43 antibody) at dilution of 1:1000. This data was developed using the same antibody clone with 12892-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of C6 cells using TDP-43 (C-terminal) Polyclonal antibody (12892-1-AP)

    WB analysis of C6 using 12892-1-AP (same clone as 12892-1-PBS)

    C6 cells were subjected to SDS PAGE followed by western blot with 12892-1-AP (TARDBP Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12892-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse brain tissue using TDP-43 (C-terminal) Polyclonal antibody (12892-1-AP)

    WB analysis of mouse brain using 12892-1-AP (same clone as 12892-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 12892-1-AP (TDP-43 (C-terminal) antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12892-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HeLa cells using TDP-43 (C-terminal) Polyclonal antibody (12892-1-AP)

    IP experiment of HeLa using 12892-1-AP (same clone as 12892-1-PBS)

    IP result of anti-TDP-43 (C-terminal) (IP:12892-1-AP, 3ug; Detection:12892-1-AP 1:1000) with HeLa cells lysate 3000ug. This data was developed using the same antibody clone with 12892-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using TDP-43 (C-terminal) Polyclonal antibody (12892-1-AP)

    IP experiment of mouse brain using 12892-1-AP (same clone as 12892-1-PBS)

    IP result of anti-TDP-43 (C-terminal) (IP:12892-1-AP, 4ug; Detection:12892-1-AP 1:1000) with mouse brain tissue lysate 3000ug. This data was developed using the same antibody clone with 12892-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat brain tissue using TDP-43 (C-terminal) Polyclonal antibody (12892-1-AP)

    IHC staining of rat brain using 12892-1-AP (same clone as 12892-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 12892-1-AP (TDP-43 (C-terminal) antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12892-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using TDP-43 (C-terminal) Polyclonal antibody (12892-1-AP)

    IHC staining of mouse brain using 12892-1-AP (same clone as 12892-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12892-1-AP (TDP-43 (C-terminal) antibody at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12892-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human gliomas tissue using TDP-43 (C-terminal) Polyclonal antibody (12892-1-AP)

    IHC staining of human gliomas using 12892-1-AP (same clone as 12892-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12892-1-AP (TDP-43 (C-terminal) antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12892-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using TDP-43 (C-terminal) Polyclonal antibody (12892-1-AP)

    IF Staining of mouse brain using 12892-1-AP (same clone as 12892-1-PBS)

    Immunofluorescent analysis ofun-fixed frozen OCT-embedded mouse brain tissue using TDP-43 (C-terminal) antibody (12892-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 12892-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using TDP-43 (C-terminal) Polyclonal antibody (12892-1-AP)

    IF Staining of mouse brain using 12892-1-AP (same clone as 12892-1-PBS)

    Immunofluorescent analysis ofun-fixed frozen OCT-embedded mouse brain tissue using TDP-43 (C-terminal) antibody (12892-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 12892-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using TDP-43 (C-terminal) Polyclonal antibody (12892-1-AP)

    IF Staining of HeLa using 12892-1-AP (same clone as 12892-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using TDP-43 (C-terminal) antibody (12892-1-AP) at dilution of 1:4000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 12892-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using TDP-43 (C-terminal) Polyclonal antibody (12892-1-AP)

    IF Staining of HeLa using 12892-1-AP (same clone as 12892-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using TDP-43 (C-terminal) antibody (12892-1-AP) at dilution of 1:2400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 12892-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of Neuro-2a cells using TDP-43 (C-terminal) Polyclonal antibody (12892-1-AP)

    IF Staining of Neuro-2a using 12892-1-AP (same clone as 12892-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Neuro-2a cells using TDP-43 (C-terminal) antibody (12892-1-AP) at dilution of 1:8000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 12892-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of Neuro-2a cells using TDP-43 (C-terminal) Polyclonal antibody (12892-1-AP)

    IF Staining of Neuro-2a using 12892-1-AP (same clone as 12892-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Neuro-2a cells using TDP-43 (C-terminal) antibody (12892-1-AP) at dilution of 1:8000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 12892-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HeLa cells using TDP-43 (C-terminal) Polyclonal antibody (12892-1-AP)

    FC experiment of HeLa using 12892-1-AP (same clone as 12892-1-PBS)

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human TDP-43 (C-terminal) (12892-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 12892-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

TDP-43 (C-terminal) Polyclonal antibody, PBS Only

TDP-43 (C-terminal) Polyclonal Antibody for WB, IHC, IF/ICC, IF-Fro, FC (Intra), IP, ELISA
Cat No. 12892-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IF-Fro, FC (Intra), IP, ELISA

Synonyms

TARDBP, TDP 43, TDP43, TDP-43, ALS10

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

12892-1-PBS targets TDP-43 (C-terminal) in WB, IHC, IF/ICC, IF-Fro, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen fusion protein 相同性解析による交差性が予測される生物種
Full Name TAR DNA binding protein
Calculated molecular weight 43 kDa
Observed molecular weight 43-45 kDa, 35 kDa
GenBank accession numberBC001487
Gene Symbol TDP-43
Gene ID (NCBI) 23435
RRIDAB_2200505
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ13148
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

Transactivation response (TAR), DNA-binding protein of 43 kDa (also known as TARDBP or TDP-43), was first isolated as a transcriptional inactivator binding to the TAR DNA element of the HIV-1 virus. Neumann et al. (2006) found that a hyperphosphorylated, ubiquitinated, and cleaved form of TARDBP, known as pathologic TDP-43, is the major component of the tau-negative and ubiquitin-positive inclusions that characterize amyotrophic lateral sclerosis (ALS) and the most common pathological subtype of frontotemporal lobar degeneration (FTLD-U). 12892-1-AP is a rabbit polyclonal antibody raised against the C-terminal amino acids of human TDP-43. This antibody recognizes the cleavage product of 20-30 kDa in addition to the native and phosphorylated forms of TDP-43. Immunohistochemical analyses of TDP-43 using this antibody detect both normal diffuse nuclear staining and insoluble inclusions in pathologic tissues. Various forms of TDP-43 exist, including 18-35 kDa of cleaved C-terminal fragments, 45-50 kDa phosphoprotein, 55 kDa glycosylated form, 75 kDa hyperphosphorylated form, and 90-300 kDa cross-linked form. (17023659,19823856,21666678,22193176)

Recently TDP-43 has been reported to be overexpressed in triple negative breast cancer (TNBC) and it may be a potential target for TNBC diagnosis and drug design. (29581274)