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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

TDP-43 Monoclonal antibody, PBS Only

TDP-43 Monoclonal Antibody for WB, FC, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Rat, Mouse

Applications

WB, FC, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1G4F5

Cat no : 66734-1-PBS

Synonyms

ALS10, TAR DNA binding protein, TAR DNA binding protein 43, TARDBP, TDP 43, TDP43, tdp-43

Validation Data Gallery

Applications:
  • WB analysis using 66734-1-Ig (same clone as 66734-1-PBS)

    WB analysis using 66734-1-Ig (same clone as 66734-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66734-1-Ig (TDP-43 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66734-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 66734-1-Ig (same clone as 66734-1-PBS)

    IHC staining of mouse brain using 66734-1-Ig (same clone as 66734-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66734-1-Ig (TDP-43 antibody) at dilution of 1:2000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66734-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 66734-1-Ig (same clone as 66734-1-PBS)

    IHC staining of mouse brain using 66734-1-Ig (same clone as 66734-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66734-1-Ig (TDP-43 antibody) at dilution of 1:2000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66734-1-PBS in a different storage buffer formulation.

  • IHC staining of human gliomas using 66734-1-Ig (same clone as 66734-1-PBS)

    IHC staining of human gliomas using 66734-1-Ig (same clone as 66734-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 66734-1-Ig (TDP-43 antibody) at dilution of 1:2000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66734-1-PBS in a different storage buffer formulation.

  • IHC staining of human gliomas using 66734-1-Ig (same clone as 66734-1-PBS)

    IHC staining of human gliomas using 66734-1-Ig (same clone as 66734-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 66734-1-Ig (TDP-43 antibody) at dilution of 1:2000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66734-1-PBS in a different storage buffer formulation.

  • IHC staining of rat liver using 66734-1-Ig (same clone as 66734-1-PBS)

    IHC staining of rat liver using 66734-1-Ig (same clone as 66734-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 66734-1-Ig (TDP-43 antibody) at dilution of 1:2000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66734-1-PBS in a different storage buffer formulation.

  • IHC staining of rat liver using 66734-1-Ig (same clone as 66734-1-PBS)

    IHC staining of rat liver using 66734-1-Ig (same clone as 66734-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 66734-1-Ig (TDP-43 antibody) at dilution of 1:2000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66734-1-PBS in a different storage buffer formulation.

  • FC experiment of SH-SY5Y using 66734-1-Ig (same clone as 66734-1-PBS)

    FC experiment of SH-SY5Y using 66734-1-Ig (same clone as 66734-1-PBS)

    1X10^6 SH-SY5Y cells were intracellularly stained with 0.20 ug/test Anti-Human TDP-43 (66734-1-Ig, Clone:1G4F5) (red) or 0.20 ug control antibody (blue) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) with dilution 1:1000. Fixed with90% MeOH. This data was developed using the same antibody clone with 66734-1-PBS in a different storage buffer formulation.

Tested Applications

Recommended dilution

ApplicationDilution
Sample-dependent, check data in validation data gallery

Product Information

66734-1-PBS targets TDP-43 in WB, FC, IHC, Indirect ELISA applications and shows reactivity with Human, Rat, Mouse samples.

Tested Reactivity Human, Rat, Mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen fusion protein 相同性解析による交差性が予測される生物種
Full Name TAR DNA binding protein
Calculated molecular weight 43 kDa
Observed molecular weight 43 kDa
GenBank accession numberBC001487
Gene symbol TARDBP
Gene ID (NCBI) 23435
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Transactivation response (TAR), DNA-binding protein of 43 kDa (also known as TARDBP or TDP-43), was first isolated as a transcriptional inactivator binding to the TAR DNA element of the HIV-1 virus. Neumann et al. (2006) found that a hyperphosphorylated, ubiquitinated, and cleaved form of TARDBP, known as pathologic TDP-43, is the major component of the tau-negative and ubiquitin-positive inclusions that characterize amyotrophic lateral sclerosis (ALS) and the most common pathological subtype of frontotemporal lobar degeneration (FTLD-U). 66734-1-Ig is a mouse monoclonal antibody recognizing the intact protein of TDP-43.