Validation Data Gallery

Filter: AllWBIPIF/ICC

WB analysis using 13370-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 13370-1-AP (TDG antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 13370-1-AP
WB result of TDG antibody (13370-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TDG transfected HEK-293 cells.
WB analysis of human brain using 13370-1-AP
human brain tissue were subjected to SDS PAGE followed by western blot with 13370-1-AP (TDG antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
WB analysis of mouse thymus using 13370-1-AP
mouse thymus tissue were subjected to SDS PAGE followed by western blot with 13370-1-AP (TDG antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 13370-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 13370-1-AP (TDG antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
WB analysis of Raji using 13370-1-AP
Raji cells were subjected to SDS PAGE followed by western blot with 13370-1-AP (TDG antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
WB analysis of mouse colon using 13370-1-AP
mouse colon tissue were subjected to SDS PAGE followed by western blot with 13370-1-AP (TDG antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
IP experiment of U-937 using 13370-1-AP
IP result of anti-TDG (IP:13370-1-AP, 3ug; Detection:13370-1-AP 1:400) with U-937 cells lysate 3000ug.
IF Staining of HeLa using 13370-1-AP
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 13370-1-AP (TDG antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Featured Product
KD/KO Validated

TDG Polyclonal antibody

TDG Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Cat No. 13370-1-AP

Publications(9)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ChIP, ELISA

Synonyms

EC:3.2.2.29, G/T mismatch-specific thymine DNA glycosylase, hTDG, thymine DNA glycosylase, Thymine-DNA glycosylase

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (9)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	Caco-2 cells, HEK-293 cells, HeLa cells, human brain tissue, mouse colon tissue, mouse thymus tissue, Raji cells, HCT-116 cells, Jurkat cells, K-562 cells, MCF-7 cells
Positive IP detected in	U-937 cells
Positive IF/ICC detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:10000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 2 publications below
WB	See 7 publications below
IHC	See 3 publications below
IP	See 1 publications below
ChIP	See 2 publications below

Product Information

13370-1-AP targets TDG in WB, IHC, IF/ICC, IP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag4190 Product name: Recombinant human TDG protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-410 aa of BC037557 Sequence: MEAENAGSYSLQQAQAFYTFPFQQLMAEAPNMAVVNEQQMPEEVPAPAPAQEPVQEAPKGRKRKPRTTEPKQPVEPKKPVESKKSGKSAKSKEKQEKITDTFKVKRKVDRFNGVSEAELLTKTLPDILTFNLDIVIIGINPGLMAAYKGHHYPGPGNHFWKCLFMSGLSEVQLNHMDDHTLPGKYGIGFTNMVERTTPGSKDLSSKEFREGGRILVQKLQKYQPRIAVFNGKCIYEIFSKEVFGVKVKNLEFGLQPHKIPDTETLCYGMPSSSARCAQFPRAQDKVHYYIKLKDLRDQLKGIERNMDVQEVQYTFDLQLAQEDAKKMAVKEEKYDPGYEAAYGGAYGENPCSSEPCGFSSNGLIESVELRGESAFSGIPNGQWMTQSFTDQIPSFSNHCGTQEQEEESHA 相同性解析による交差性が予測される生物種
Full Name	thymine-DNA glycosylase
Calculated molecular weight	410 aa, 46 kDa
Observed molecular weight	55-60 kDa
GenBank accession number	BC037557
Gene Symbol	TDG
Gene ID (NCBI)	6996
RRID	AB_2271704
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q13569
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

TDG belongs to the TDG/mug DNA glycosylase family. TDG corrects G/T mispairs to G/C pairs. It is capable of hydrolyzing the carbon-nitrogen bond between the sugar-phosphate backbone of the DNA and a mispaired thymine. In addition to the G/T, it can remove thymine also from C/T and T/T mispairs in the order G/T >> C/T > T/T. It has no detectable activity on apyrimidinic sites and does not catalyze the removal of thymine from A/T pairs or from single-stranded DNA. It can also remove uracil and 5-bromouracil from mispairs with guanine. RNF4 interacts with and requires the base excision repair enzymes TDG and APE1 for active demethylation (PMID:20696907). TDG is modified by SUMO-1 and SUMO-2/3.The molecular weight of non-modified TDG is 46 kDa and modified TDG is 55-60 kDa.

Protocols

Product Specific Protocols
IF protocol for TDG antibody 13370-1-AP	Download protocol
IP protocol for TDG antibody 13370-1-AP	Download protocol
WB protocol for TDG antibody 13370-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB,IP	J Cell Biol Oxidative stress-induced assembly of PML nuclear bodies controls sumoylation of partner proteins. Authors - Umut Sahin View Article
human	WB	Proc Natl Acad Sci U S A Identification of RING finger protein 4 (RNF4) as a modulator of DNA demethylation through a functional genomics screen. Authors - Hu Xiaoyi V XV KD Validated View Article
human	WB	Int J Biol Sci TDG suppresses the migration and invasion of human colon cancer cells via the DNMT3A/TIMP2 axis. Authors - Jiyu Miao View Article
human	IHC,ChIP	J Biol Chem Thymine DNA Glycosylase is a Positive Regulator of Wnt Signaling in Colorectal Cancer. Authors - Xuehe Xu View Article
human	WB	J Biol Chem CRL4Cdt2 E3 Ubiquitin Ligase and PCNA Cooperate to Degrade Thymine DNA Glycosylase in S-phase. Authors - Etsuko Shibata View Article
human	WB	Cell Death Dis The HOTAIRM1/miR-107/TDG axis regulates papillary thyroid cancer cell proliferation and invasion. Authors - Dan Li View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 13370-1-AP

WB analysis of HEK-293 using 13370-1-AP

WB analysis of human brain using 13370-1-AP

WB analysis of mouse thymus using 13370-1-AP

WB analysis of HeLa using 13370-1-AP

WB analysis of Raji using 13370-1-AP

WB analysis of mouse colon using 13370-1-AP

IP experiment of U-937 using 13370-1-AP

IF Staining of HeLa using 13370-1-AP

TDG Polyclonal antibody

TDG Polyclonal Antibody for WB, IF/ICC, IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Oxidative stress-induced assembly of PML nuclear bodies controls sumoylation of partner proteins.

Identification of RING finger protein 4 (RNF4) as a modulator of DNA demethylation through a functional genomics screen.

TDG suppresses the migration and invasion of human colon cancer cells via the DNMT3A/TIMP2 axis.

Thymine DNA Glycosylase is a Positive Regulator of Wnt Signaling in Colorectal Cancer.

CRL4Cdt2 E3 Ubiquitin Ligase and PCNA Cooperate to Degrade Thymine DNA Glycosylase in S-phase.

The HOTAIRM1/miR-107/TDG axis regulates papillary thyroid cancer cell proliferation and invasion.