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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TCOF1 Recombinant antibody (84914-4-RR)

    WB analysis using 84914-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84914-4-RR (TCOF1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A549 cells using TCOF1 Recombinant antibody (84914-4-RR)

    WB analysis of A549 using 84914-4-RR

    A549 cells were subjected to SDS PAGE followed by western blot with 84914-4-RR (TCOF1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using TCOF1 Recombinant antibody (84914-4-RR)

    IF Staining of MCF-7 using 84914-4-RR

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using TCOF1 antibody (84914-4-RR, Clone: 242201E12 ) at dilution of 1:600 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

TCOF1 Recombinant antibody

TCOF1 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 84914-4-RR
Clone No.242201E12

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

242201E12, MFD1, treacle

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inJurkat cells, A549 cells, HEK-293T cells
Positive IF/ICC detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:300-1:1200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84914-4-RR targets TCOF1 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen TCOF1 fusion protein Ag1447 相同性解析による交差性が予測される生物種
Full Name Treacher Collins-Franceschetti syndrome 1
Calculated molecular weight 97 kDa, 144 kDa, 152 kDa
Observed molecular weight220-240 kDa
GenBank accession numberBC014559
Gene Symbol TCOF1
Gene ID (NCBI) 6949
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ13428
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TCOF1 also known as treacle, is a nucleolar protein with a LIS1 homology domain. TCOF1 is involved in ribosomal DNA gene transcription by interacting with upstream binding factor (UBF). Mutations in TCOF1 have been associated with Treacher Collins syndrome, a disorder which includes abnormal craniofacial development(PMID: 31218872)

Protocols

Product Specific Protocols
WB protocol for TCOF1 antibody 84914-4-RRDownload protocol
IF protocol for TCOF1 antibody 84914-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols