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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TCN2 Polyclonal antibody (12157-1-AP)

    WB analysis using 12157-1-AP (same clone as 12157-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 12157-1-AP (TCN2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12157-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse small intestine tissue using TCN2 Polyclonal antibody (12157-1-AP)

    WB analysis of mouse small intestine using 12157-1-AP (same clone as 12157-1-PBS)

    mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 12157-1-AP (TCN2 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12157-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat liver tissue using TCN2 Polyclonal antibody (12157-1-AP)

    WB analysis of rat liver using 12157-1-AP (same clone as 12157-1-PBS)

    rat liver tissue were subjected to SDS PAGE followed by western blot with 12157-1-AP (TCN2 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12157-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse small intestine tissue using TCN2 Polyclonal antibody (12157-1-AP)

    IHC staining of mouse small intestine using 12157-1-AP (same clone as 12157-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 12157-1-AP (TCN2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12157-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse small intestine tissue using TCN2 Polyclonal antibody (12157-1-AP)

    IHC staining of mouse small intestine using 12157-1-AP (same clone as 12157-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 12157-1-AP (TCN2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12157-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using TCN2 Polyclonal antibody (12157-1-AP)

    IHC staining of human pancreas cancer using 12157-1-AP (same clone as 12157-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 12157-1-AP (TCN2 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12157-1-PBS in a different storage buffer formulation.

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TCN2 Polyclonal antibody, PBS Only

TCN2 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 12157-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

12157-1-PBS targets TCN2 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TCN2 fusion protein Ag2825 相同性解析による交差性が予測される生物種
Full Name transcobalamin II; macrocytic anemia
Calculated molecular weight 400 aa, 44 kDa
Observed molecular weight 48 kDa
GenBank accession numberBC011239
Gene Symbol TCN2
Gene ID (NCBI) 6948
RRIDAB_2303252
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP20062
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

TCN2 (Transcobalamin-2) is a vitamin B12 (cobalamin; Cbl) binding and transport protein in plasma that facilitates the cellular uptake of the vitamin. TCN2 is secreted in a constitutive fashion in most cells. Plasma TCN2 levels have been reported to be elevated in a number of diverse human diseases. Defects in TCN2 are cause of transcobalamin-2 deficiency. (10714245)