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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

TCN1 Polyclonal antibody

TCN1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 16078-1-AP

Synonyms

TC 1, TC I, TC1, TCI, TCN1, Transcobalamin 1, Transcobalamin I

Validation Data Gallery

Applications:
  • WB analysis of human saliva using 16078-1-AP

    WB analysis of human saliva using 16078-1-AP

    human saliva were subjected to SDS PAGE followed by western blot with 16078-1-AP (TCN1 Antibody) at dilution of 1:2000 incubated at 4 degree celsius over night.

  • IHC staining of human testis using 16078-1-AP

    IHC staining of human testis using 16078-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 16078-1-AP (TCN1 Antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human testis using 16078-1-AP

    IHC staining of human testis using 16078-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 16078-1-AP (TCN1 Antibody) at dilution of 1:50 (under 40x lens).

  • IHC staining of human tonsillitis using 16078-1-AP

    IHC staining of human tonsillitis using 16078-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 16078-1-AP (TCN1 Antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human tonsillitis using 16078-1-AP

    IHC staining of human tonsillitis using 16078-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 16078-1-AP (TCN1 Antibody) at dilution of 1:50 (under 40x lens).

Tested Applications

Positive WB detected inhuman saliva tissue
Positive IHC detected inhuman testis tissue, human tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 16078-1-AP is TCN1 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TCN1 fusion protein Ag9072 相同性解析による交差性が予測される生物種
Full Name transcobalamin I (vitamin B12 binding protein, R binder family)
Calculated molecular weight 433 aa, 48 kDa
Observed molecular weight 65-70 kDa
GenBank accession numberBC018632
Gene symbol TCN1
Gene ID (NCBI) 6947
RRIDAB_2878215
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for TCN1 antibody 16078-1-APDownload protocol
IHC protocol for TCN1 antibody 16078-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols