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Species Reactivity
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

TCFL5 Polyclonal antibody

TCFL5 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 29404-1-AP

Synonyms

CHA, Cha transcription factor, E2BP 1, E2BP1, Figlb, HPV 16 E2 binding protein 1, TCFL5

Validation Data Gallery

Applications:
  • WB analysis of mouse testis using 29404-1-AP

    WB analysis of mouse testis using 29404-1-AP

    Mouse testis tissue were subjected to SDS PAGE followed by western blot with 29404-1-AP (TCFL5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse testis using 29404-1-AP

    IHC staining of mouse testis using 29404-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 29404-1-AP (TCFL5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with TCFL5 antibody included
  • IHC staining of mouse testis using 29404-1-AP

    IHC staining of mouse testis using 29404-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 29404-1-AP (TCFL5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with TCFL5 antibody included

Tested Applications

Positive WB detected inmouse testis tissue
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy TCFL5 Ready-To-Use IHC Kit. TCFL5 primary antibody included.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 1 publications below

Product Information

29404-1-AP targets TCFL5 in WB, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TCFL5 fusion protein Ag13479 相同性解析による交差性が予測される生物種
Full Name transcription factor-like 5 (basic helix-loop-helix)
Calculated molecular weight 483 aa, 52 kDa
Observed molecular weight 52-60 kDa
GenBank accession numberBC046933
Gene symbol TCFL5
Gene ID (NCBI) 10732
RRIDAB_2918297
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for TCFL5 antibody 29404-1-APDownload protocol
IHC protocol for TCFL5 antibody 29404-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
WB

Cell Signal

TCFL5 knockdown sensitizes DLBCL to doxorubicin treatment via regulation of GPX4

Authors - Xueying Lu
View Article