Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse liver tissue, human liver tissue, human brain tissue, human heart tissue, HepG2 cells, mouse kidney tissue, mouse heart tissue, mouse placenta tissue, rat liver tissue, HEK-293 cells |
| Positive IP detected in | mouse liver tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 3 publications below |
| WB | See 34 publications below |
| IHC | See 5 publications below |
| IF | See 11 publications below |
| IP | See 2 publications below |
| CoIP | See 3 publications below |
| ChIP | See 3 publications below |
Product Information
13838-1-AP targets TCF4/TCF7L2 in WB, IHC, IF, IP, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, bovine |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag4786 Product name: Recombinant human TCF7L2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-355 aa of BC032656 Sequence: MPQLNGGGGDDLGANDELISFKDEGEQEEKSSENSSAERDLADVKSSLVNESETNQNSSSDSEAERRPPPRSESFRDKSRESLEEAAKRQDGGLFKGPPYPGYPFIMIPDLTSPYLPNGSLSPTARTYLQMKWPLLDVQAGSLQSRQALKDARSPSPAHIVSNKVPVVQHPHHVHPLTPLITYSNEHFTPGNPPPHLPADVDPKTGIPRPPHPPDISPYYPLSPGTVGQIPHPLGWQGQPVYPITTGGFRHPYPTALTVNASMSRFPPHMVPPHHTLHTTGIPHPAIVTPTVKQESSQSDVGSLHSSKHQDSKKEEEKKKPHIKKPLNAFMLYMKEMRAKVVAECTLKESAAINQ 相同性解析による交差性が予測される生物種 |
| Full Name | transcription factor 7-like 2 (T-cell specific, HMG-box) |
| Calculated molecular weight | 619 aa, 68 kDa |
| Observed molecular weight | 68 kDa |
| GenBank accession number | BC032656 |
| Gene Symbol | TCF4 |
| Gene ID (NCBI) | 6934 |
| RRID | AB_2199812 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9NQB0 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
TCF7L2 encodes an HMG-box transcription factor and key effector of canonical Wnt/β-catenin signaling, regulating development and tissue homeostasis. It is the strongest genetic susceptibility locus for type 2 diabetes. Alternative splicing produces functionally distinct isoforms, and TCF7L2 variants have also been linked to Crohn's disease and cancer, indicating pleiotropic roles in disease.
Protocols
| Product Specific Protocols | |
|---|---|
| IP protocol for TCF4/TCF7L2 antibody 13838-1-AP | Download protocol |
| WB protocol for TCF4/TCF7L2 antibody 13838-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell Mol Immunol TRIM27 maintains gut homeostasis by promoting intestinal stem cell self-renewal | ||
Drug Resist Updat ATP-binding cassette subfamily A member 5 suppresses pancreatic ductal adenocarcinoma progression and chemoresistance by promoting β-catenin ubiquitin-dependent degradation. | ||
Bone Res Single-cell sequencing reveals a senescent immune landscape in bone marrow lesions inducing articular cartilage damage in osteoarthritis. | ||
Nat Microbiol A single-cell transcriptomic atlas reveals senescence and inflammation in the post-tuberculosis human lung | ||
J Control Release Nanoceria drive stem cell hepatic differentiation and microenvironment remodeling for acute liver failure therapy. | ||
Pharmacol Res Targeting aberrant glycosylation to modulate microglial response and improve cognition in models of Alzheimer's disease |