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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TCERG1 Polyclonal antibody (21858-1-AP)

    WB analysis using 21858-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 21858-1-AP (TCERG1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using TCERG1 Polyclonal antibody (21858-1-AP)

    WB analysis of HEK-293 using 21858-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 21858-1-AP (TCERG1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using TCERG1 Polyclonal antibody (21858-1-AP)

    WB analysis of HeLa using 21858-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 21858-1-AP (TCERG1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of NIH/3T3 cells using TCERG1 Polyclonal antibody (21858-1-AP)

    WB analysis of NIH/3T3 using 21858-1-AP

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 21858-1-AP (TCERG1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using TCERG1 Polyclonal antibody (21858-1-AP)

    WB analysis using 21858-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 21858-1-AP (TCERG1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human brain tissue using TCERG1 Polyclonal antibody (21858-1-AP)

    IHC staining of human brain using 21858-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 21858-1-AP (TCERG1 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human brain tissue using TCERG1 Polyclonal antibody (21858-1-AP)

    IHC staining of human brain using 21858-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 21858-1-AP (TCERG1 antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using TCERG1 Polyclonal antibody (21858-1-AP)

    IF Staining of HeLa using 21858-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 21858-1-AP (TCERG1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

TCERG1 Polyclonal antibody

TCERG1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 21858-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

CA150, TAF2S, TCERG1, Transcription factor CA150

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells, NIH/3T3 cells
Positive IHC detected inhuman brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

21858-1-AP targets TCERG1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag16482

Product name: Recombinant human TCERG1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 728-1077 aa of BC111727

Sequence: EFAAKHAKDSRFKAIEKMKDREALFNEFVAAARKKEKEDSKTRGEKIKSDFFELLSNHHLDSQSRWSKVKDKVESDPRYKAVDSSSMREDLFKQYIEKIAKNLDSEKEKELERQARIEASLREREREVQKARSEQTKEIDREREQHKREEAIQNFKALLSDMVRSSDVSWSDTRRTLRKDHRWESGSLLEREEKEKLFNEHIEALTKKKREHFRQLLDETSAITLTSTWKEVKKIIKEDPRCIKFSSSDRKKQREFEEYIRDKYITAKADFRTLLKETKFITYRSKKLIQESDQHLKDVEKILQNDKRYLVLDCVPEERRKLIVAYVDDLDRRGPPPPPTASEPTRRSTK

相同性解析による交差性が予測される生物種
Full Name transcription elongation regulator 1
Calculated molecular weight 1098 aa, 124 kDa
Observed molecular weight 150 kDa
GenBank accession numberBC111727
Gene Symbol TCERG1
Gene ID (NCBI) 10915
RRIDAB_11183756
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDO14776
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IF protocol for TCERG1 antibody 21858-1-APDownload protocol
IHC protocol for TCERG1 antibody 21858-1-APDownload protocol
WB protocol for TCERG1 antibody 21858-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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