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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

TCERG1 Polyclonal antibody

TCERG1 Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 21858-1-AP

Synonyms

CA150, TAF2S, TCERG1, Transcription factor CA150

Validation Data Gallery

Applications:
  • WB analysis using 21858-1-AP

    WB analysis using 21858-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 21858-1-AP (TCERG1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 21858-1-AP

    WB analysis of HeLa using 21858-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 21858-1-AP (TCERG1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of NIH/3T3 using 21858-1-AP

    WB analysis of NIH/3T3 using 21858-1-AP

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 21858-1-AP (TCERG1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of HEK-293 using 21858-1-AP

    WB analysis of HEK-293 using 21858-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 21858-1-AP (TCERG1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • WB analysis using 21858-1-AP

    WB analysis using 21858-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 21858-1-AP (TCERG1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of human brain using 21858-1-AP

    IHC staining of human brain using 21858-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 21858-1-AP (TCERG1 antibody) at dilution of 1:50 (under 10x lens).

    Ready-to-use IHC kits available with TCERG1 antibody included
  • IHC staining of human brain using 21858-1-AP

    IHC staining of human brain using 21858-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 21858-1-AP (TCERG1 antibody) at dilution of 1:50 (under 40x lens).

    Ready-to-use IHC kits available with TCERG1 antibody included
  • IF Staining of HeLa using 21858-1-AP

    IF Staining of HeLa using 21858-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 21858-1-AP (TCERG1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells, NIH/3T3 cells
Positive IHC detected inhuman brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy TCERG1 Ready-To-Use IHC Kit. TCERG1 primary antibody included.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 21858-1-AP is TCERG1 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TCERG1 fusion protein Ag16482 相同性解析による交差性が予測される生物種
Full Name transcription elongation regulator 1
Calculated molecular weight 1098 aa, 124 kDa
Observed molecular weight 150 kDa
GenBank accession numberBC111727
Gene symbol TCERG1
Gene ID (NCBI) 10915
RRIDAB_11183756
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for TCERG1 antibody 21858-1-APDownload protocol
IHC protocol for TCERG1 antibody 21858-1-APDownload protocol
IF protocol for TCERG1 antibody 21858-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols