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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

TCEB3 Polyclonal antibody

TCEB3 Polyclonal Antibody for WB, IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Cat no : 10502-1-AP

Synonyms

EloA, Elongin 110 kDa subunit, Elongin A, SIII, SIII p110, TCEB3, TCEB3A

Validation Data Gallery

Applications:
  • WB analysis of mouse liver using 10502-1-AP

    WB analysis of mouse liver using 10502-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 10502-1-AP (TCEB3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis using 10502-1-AP

    WB analysis using 10502-1-AP

    Jurkat cell lysates were subjected to SDS PAGE followed by western blot with 10502-1-AP (TCEB3 antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours.

  • WB analysis of mouse brain using 10502-1-AP

    WB analysis of mouse brain using 10502-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 10502-1-AP (TCEB3 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 10502-1-AP

    IF Staining of HeLa using 10502-1-AP

    Immunofluorescent analysis of Hela cells, using TCEB3 antibody 10502-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

Tested Applications

Positive WB detected inmouse liver tissue, mouse brain tissue, Jurkat cells
Positive IF detected inHela cells
Planning an IHC experiment? We recommend our IHCeasy ELOA Ready-To-Use IHC Kit. ELOA primary antibody included.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

10502-1-AP targets TCEB3 in WB, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TCEB3 fusion protein Ag0778 相同性解析による交差性が予測される生物種
Full Name transcription elongation factor B (SIII), polypeptide 3 (110kDa, elongin A)
Calculated molecular weight 110 kDa
Observed molecular weight 110 kDa
GenBank accession numberBC002883
Gene symbol TCEB3
Gene ID (NCBI) 6924
RRIDAB_2201278
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for TCEB3 antibody 10502-1-APDownload protocol
IF protocol for TCEB3 antibody 10502-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols