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Species Reactivity
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Species Reactivity
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Conjugate
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Conjugate
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

TCEA3 Polyclonal antibody

TCEA3 Polyclonal Antibody for IF, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, IF, ELISA

Conjugate

Unconjugated

Cat no : 17158-1-AP

Synonyms

TCEA3, TFIIS, TFIIS.H, TFIISH

Validation Data Gallery

Applications:
  • WB analysis using 17158-1-AP

    WB analysis using 17158-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17158-1-AP (TCEA3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IP experiment of HeLa using 17158-1-AP

    IP experiment of HeLa using 17158-1-AP

    IP result of anti-TCEA3 (IP:17158-1-AP, 4ug; Detection:17158-1-AP 1:500) with HeLa cells lysate 520ug.

  • IF Staining of HeLa using 17158-1-AP

    IF Staining of HeLa using 17158-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 17158-1-AP (TCEA3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHeLa cells, HepG2 cells
Positive IP detected inHeLa cells
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 1 publications below

Product Information

17158-1-AP targets TCEA3 in WB, IP, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TCEA3 fusion protein Ag10404 相同性解析による交差性が予測される生物種
Full Name transcription elongation factor A (SII), 3
Calculated molecular weight 348 aa, 39 kDa
Observed molecular weight 39 kDa
GenBank accession numberBC041613
Gene symbol TCEA3
Gene ID (NCBI) 6920
RRIDAB_2240352
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for TCEA3 antibody 17158-1-APDownload protocol
IF protocol for TCEA3 antibody 17158-1-APDownload protocol
IP protocol for TCEA3 antibody 17158-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Med Sci Monit

TCEA3 Attenuates Gastric Cancer Growth by Apoptosis Induction.

Authors - Jia Li
View Article