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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TCEA3 Polyclonal antibody (17158-1-AP)

    WB analysis using 17158-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17158-1-AP (TCEA3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using TCEA3 Polyclonal antibody (17158-1-AP)

    IP experiment of HeLa using 17158-1-AP

    IP result of anti-TCEA3 (IP:17158-1-AP, 4ug; Detection:17158-1-AP 1:500) with HeLa cells lysate 520ug.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using TCEA3 Polyclonal antibody (17158-1-AP)

    IF Staining of HeLa using 17158-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 17158-1-AP (TCEA3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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TCEA3 Polyclonal antibody

TCEA3 Polyclonal Antibody for WB, IF/ICC, IP, ELISA
Cat No. 17158-1-AP
Publications(3)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, IP, ELISA

Synonyms

Transcription elongation factor S-II protein 3, TFIISH, TFIIS.H, TFIIS

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, HepG2 cells
Positive IP detected inHeLa cells
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 3 publications below

Product Information

17158-1-AP targets TCEA3 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TCEA3 fusion protein Ag10404 相同性解析による交差性が予測される生物種
Full Name transcription elongation factor A (SII), 3
Calculated molecular weight 348 aa, 39 kDa
Observed molecular weight 39 kDa
GenBank accession numberBC041613
Gene Symbol TCEA3
Gene ID (NCBI) 6920
RRIDAB_2240352
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO75764
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for TCEA3 antibody 17158-1-APDownload protocol
IF protocol for TCEA3 antibody 17158-1-APDownload protocol
IP protocol for TCEA3 antibody 17158-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Med Sci Monit

TCEA3 Attenuates Gastric Cancer Growth by Apoptosis Induction.

Authors - Jia Li
View Article
humanWB

J Gene Med

Oncogenic tRNA-derived fragment tRF-Leu-CAG promotes tumorigenesis of lung cancer via targeting TCEA3 and increasing autophagy

Authors - Fan Wu
View Article
humanWB

J Cell Mol Med

Linoleic Acid Enhances Renal Tubular Epithelial Cells Autophagy Caused by Calcium Oxalate Monohydrate Crystals

Authors - Weiming Ma
View Article