Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse heart tissue, mouse skeletal muscle tissue, rat skeletal muscle tissue |
| Positive IHC detected in | mouse skeletal muscle tissue, mouse heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
16104-1-AP targets Telethonin in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag9014 Product name: Recombinant human TCAP protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-167 aa of BC012628 Sequence: MATSELSCEVSEENCERREAFWAEWKDLTLSTRPEEGCSLHEEDTQRHETYHQQGQCQVLVQRSPWLMMRMGILGRGLQEYQLPYQRVLPLPIFTPAKMGATKEEREDTPIQLQELLALETALGGQCVDRQEVAEITKQLPPVVPVSKPGALRRSLSRSMSQEAQRG 相同性解析による交差性が予測される生物種 |
| Full Name | titin-cap (telethonin) |
| Calculated molecular weight | 167 aa, 19 kDa |
| Observed molecular weight | 19-21 kDa |
| GenBank accession number | BC012628 |
| Gene Symbol | TCAP |
| Gene ID (NCBI) | 8557 |
| RRID | AB_3741877 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O15273 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Telethonin, also known as TCAP, is a sarcomeric protein found exclusively in striated and cardiac muscle, where it localizes to the periphery of Z discs that define the border of the sarcomere. It binds to the N-terminus of myoglobin, effectively increasing its mechanical resistance. Mutations in this gene are associated with limb-girdle muscular dystrophy type 2G(PMID: 19679566).
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for Telethonin antibody 16104-1-AP | Download protocol |
| WB protocol for Telethonin antibody 16104-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |