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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Telethonin Polyclonal antibody (16104-1-AP)

    WB analysis using 16104-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16104-1-AP (Telethonin antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse heart tissue using Telethonin Polyclonal antibody (16104-1-AP)

    IHC staining of mouse heart using 16104-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 16104-1-AP (Telethonin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using Telethonin Polyclonal antibody (16104-1-AP)

    IHC staining of mouse skeletal muscle using 16104-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 16104-1-AP (Telethonin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Telethonin Polyclonal antibody

Telethonin Polyclonal Antibody for WB, IHC, ELISA
Cat No. 16104-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

TCAP, CMD1N

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse heart tissue, mouse skeletal muscle tissue, rat skeletal muscle tissue
Positive IHC detected inmouse skeletal muscle tissue, mouse heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16104-1-AP targets Telethonin in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Telethonin fusion protein Ag9014 相同性解析による交差性が予測される生物種
Full Name titin-cap (telethonin)
Calculated molecular weight 167 aa, 19 kDa
Observed molecular weight 19 kDa
GenBank accession numberBC012628
Gene Symbol TCAP
Gene ID (NCBI) 8557
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO15273
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Telethonin, also known as TCAP, is a sarcomeric protein found exclusively in striated and cardiac muscle, where it localizes to the periphery of Z discs that define the border of the sarcomere. It binds to the N-terminus of myoglobin, effectively increasing its mechanical resistance. Mutations in this gene are associated with limb-girdle muscular dystrophy type 2G(PMID: 19679566).

Protocols

Product Specific Protocols
WB protocol for Telethonin antibody 16104-1-APDownload protocol
IHC protocol for Telethonin antibody 16104-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols