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Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using TBX6 Polyclonal antibody (12447-1-AP)

    WB analysis of HeLa using 12447-1-AP (same clone as 12447-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 12447-1-AP (TBX6 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12447-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse lung tissue using TBX6 Polyclonal antibody (12447-1-AP)

    WB analysis of mouse lung using 12447-1-AP (same clone as 12447-1-PBS)

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 12447-1-AP (TBX6 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12447-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of A375 cells using TBX6 Polyclonal antibody (12447-1-AP)

    WB analysis of A375 using 12447-1-AP (same clone as 12447-1-PBS)

    A375 cells were subjected to SDS PAGE followed by western blot with 12447-1-AP (TBX6 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12447-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human placenta tissue using TBX6 Polyclonal antibody (12447-1-AP)

    WB analysis of human placenta using 12447-1-AP (same clone as 12447-1-PBS)

    human placenta tissue were subjected to SDS PAGE followed by western blot with 12447-1-AP (TBX6 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12447-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Raji cells using TBX6 Polyclonal antibody (12447-1-AP)

    WB analysis of Raji using 12447-1-AP (same clone as 12447-1-PBS)

    Raji cells were subjected to SDS PAGE followed by western blot with 12447-1-AP (TBX6 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12447-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of A549 cells using TBX6 Polyclonal antibody (12447-1-AP)

    WB analysis of A549 using 12447-1-AP (same clone as 12447-1-PBS)

    A549 cells were subjected to SDS PAGE followed by western blot with 12447-1-AP (TBX6 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12447-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human lung tissue using TBX6 Polyclonal antibody (12447-1-AP)

    WB analysis of human lung using 12447-1-AP (same clone as 12447-1-PBS)

    human lung tissue were subjected to SDS PAGE followed by western blot with 12447-1-AP (TBX6 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12447-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of human placenta tissue using TBX6 Polyclonal antibody (12447-1-AP)

    IP experiment of human placenta using 12447-1-AP (same clone as 12447-1-PBS)

    IP result of anti-TBX6 (IP:12447-1-AP, 4ug; Detection:12447-1-AP 1:500) with human placenta tissue lysate 2800ug. This data was developed using the same antibody clone with 12447-1-PBS in a different storage buffer formulation.

TBX6 Polyclonal antibody, PBS Only

TBX6 Polyclonal Antibody for WB, IP, Indirect ELISA
Cat No. 12447-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

12447-1-PBS targets TBX6 in WB, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag3050

Product name: Recombinant human TBX6 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 87-436 aa of BC026031

Sequence: SLPGVSLSLENRELWKEFSSVGTEMIITKAGRRMFPACRVSVTGLDPEARYLFLLDVIPVDGARYRWQGRRWEPSGKAEPRLPDRVYIHPDSPATGAHWMRQPVSFHRVKLTNSTLDPHGHLILHSMHKYQPRIHLVRAAQLCSQHWGGMASFRFPETTFISVTAYQNPQITQLKIAANPFAKGFRENGRNCKRERDARVKRKLRGPEPAATEAYGSGDTPGGPCDSTLGGDIRESDPEQAPAPGEATAAPAPLCGGPSAEAYLLHPAAFHGAPSHLPTRSPSFPEAPDSGRSAPYSAAFLELPHGSGGSGYPAAPPAVPFAPHFLQGGPFPLPYTAPGGYLDVGSKPMY

相同性解析による交差性が予測される生物種
Full Name T-box 6
Calculated molecular weight 436 aa, 47 kDa
Observed molecular weight 58 kDa
GenBank accession numberBC026031
Gene Symbol TBX6
Gene ID (NCBI) 6911
RRIDAB_2200836
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDO95947
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

TBX6, also named as T-box transcription factor TBX6, is a 436 amino acid protein, which localizes in the nucleus and forms a dimeric complex with DNA. T-box transcription factor that plays an essential role in the determination of the fate of axial stem cells: neural vs mesodermal. The calculated molecular weight of TBX6 is 47 kDa, but the modified TBX6 is about 58 kDa.

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