Validation Data Gallery
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Tested Applications
| Positive WB detected in | CTLL-2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
83804-6-RR targets TBX21/T-bet in WB, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen | TBX21/T-bet fusion protein Ag4618 相同性解析による交差性が予測される生物種 |
| Full Name | T-box 21 |
| Calculated molecular weight | 535 aa, 58 kDa |
| Observed molecular weight | 60-65 kDa |
| GenBank accession number | BC039739 |
| Gene Symbol | TBX21/T-bet |
| Gene ID (NCBI) | 30009 |
| ENSEMBL Gene ID | ENSG00000073861 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purfication |
| UNIPROT ID | Q9UL17 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
TBX21 is a transcription factor that drives the Th1 immune response primarily through promoting expression of the IFN-γ gene. TBX21 initiates TH1 lineage development from naive TH precursor cells both by activating TH1 genetic programs and by repressing the opposing TH2 programs. It has also been shown to play an influential role in inflammatory processes such as inflammatory bowel disease, autoimmune diseases such as rheumatoid arthritis, and immune-mediated conditions like asthma.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for TBX21/T-bet antibody 83804-6-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |