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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TBX20 Recombinant antibody (83414-5-RR)

    WB analysis using 83414-5-RR (same clone as 83414-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83414-5-RR (TBX20 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83414-5-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of k562 using TBX20 Recombinant antibody (83414-5-RR)

    FC experiment of k562 using 83414-5-RR (same clone as 83414-5-PBS)

    1x10^6 k562 were intracellularly stained with 0.25 ug Tbx20 Recombinant Antibody (83414-5-RR, Clone:240385C12) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83414-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83414-5-RR

    Affinity and Kinetic Characterization of 83414-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83414-5-RR against Human TBX20 were performed. The affinity constant is 30 nM.

TBX20 Recombinant monoclonal antibody, PBS Only

TBX20 Uni-rAb® Recombinant Antibody for WB, FC (Intra), ELISA
Cat No. 83414-5-PBS
Clone No.240385C12

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, FC (Intra), ELISA

Synonyms

TBX 20, T-box transcription factor TBX20, T-box protein 20, T box 20, ASD4

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83414-5-PBS targets TBX20 in WB, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag18501

Product name: Recombinant human TBX20 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-119 aa of BC120946

Sequence: MEFTASPKPQLSSRANAFSIAALMSSGGSKEKEATENTIKPLEQFVEKSSCAQPLGELTSLDAHGEFGGGSGSSPSSSSLCTEPLIPTTPIIPSEEMAKIACSLETKELWDKFHELGTE

相同性解析による交差性が予測される生物種
Full Name T-box 20
Calculated molecular weight 297 aa, 33 kDa
Observed molecular weight50-60 kDa
GenBank accession numberBC120946
Gene Symbol TBX20
Gene ID (NCBI) 57057
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purfication
UNIPROT IDQ9UMR3
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Tbx20 is a transcription factor that is essential for proper heart development in a growing fetus. Any mutations in this gene can result in various forms of congenital heart disease.

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