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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TBR1 Monoclonal antibody (66564-1-Ig)

    WB analysis using 66564-1-Ig (same clone as 66564-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66564-1-Ig (TBR1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 66564-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of pig cerebellum tissue using TBR1 Monoclonal antibody (66564-1-Ig)

    WB analysis of pig cerebellum using 66564-1-Ig (same clone as 66564-1-PBS)

    pig cerebellum tissue were subjected to SDS PAGE followed by western blot with 66564-1-Ig (TBR1 antibody) at dilution of 1:2000 incubated at 4 degree celsius over night. This data was developed using the same antibody clone with 66564-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat cerebellum tissue using TBR1 Monoclonal antibody (66564-1-Ig)

    WB analysis of rat cerebellum using 66564-1-Ig (same clone as 66564-1-PBS)

    rat cerebellum tissue were subjected to SDS PAGE followed by western blot with 66564-1-Ig (TBR1 antibody) at dilution of 1:2000 incubated at 4 degree celsius over night. This data was developed using the same antibody clone with 66564-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using TBR1 Monoclonal antibody (66564-1-Ig)

    IHC staining of mouse brain using 66564-1-Ig (same clone as 66564-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66564-1-Ig (TBR1 antibody) at dilution of 1:200 (under 4x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66564-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using TBR1 Monoclonal antibody (66564-1-Ig)

    IHC staining of mouse brain using 66564-1-Ig (same clone as 66564-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66564-1-Ig (TBR1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66564-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using TBR1 Monoclonal antibody (66564-1-Ig)

    IHC staining of mouse brain using 66564-1-Ig (same clone as 66564-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66564-1-Ig (TBR1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66564-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using TBR1 Monoclonal antibody (66564-1-Ig)

    IF Staining of mouse brain using 66564-1-Ig (same clone as 66564-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using TBR1 antibody (66564-1-Ig, Clone: 5C3A5 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66564-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using TBR1 Monoclonal antibody (66564-1-Ig)

    IF Staining of mouse brain using 66564-1-Ig (same clone as 66564-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using TBR1 antibody (66564-1-Ig, Clone: 5C3A5 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66564-1-PBS in a different storage buffer formulation.

TBR1 Monoclonal antibody, PBS Only

TBR1 Monoclonal Antibody for WB, IHC, IF-P, ELISA
Cat No. 66564-1-PBS
Clone No.5C3A5

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF-P, ELISA

Synonyms

T box brain protein 1, T box, brain, 1, T brain 1, T-box brain protein 1, TBR 1

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66564-1-PBS targets TBR1 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag15262

Product name: Recombinant human TBR1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-196 aa of BC104844

Sequence: MQLEHCLSPSIMLSKKFLNVSSSYPHSGGSELVLHDHPIISTTDNLERSSPLKKITRGMTNQSDTDNFPDSKDSPGDVQRSKLSPVLDGVSELRHSFDGSAADRYLLSQSSQPQSAATAPSAMFPYPGQHGPAHPAFSIGSPSRYMAHHPVITNGAYNSLLSNSSPQGYPTAGYPYPQQYGHSYQGAPFYQFSSTQ

相同性解析による交差性が予測される生物種
Full Name T-box, brain, 1
Calculated molecular weight 682 aa, 74 kDa
Observed molecular weight 74 kDa
GenBank accession numberBC104844
Gene Symbol TBR1
Gene ID (NCBI) 10716
RRIDAB_2881925
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein G purification
UNIPROT IDQ16650
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

TBR1, also named T-box brain protein 1, is a 682 amino acid protein, which contains one T-box DNA-binding domain and localizes in the nucleus. TBR1 is expressed in the brain and as a transcriptional regulator is involved in developmental processes. TBR1 is required for normal brain development.

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