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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse testis tissue using TBPL1 Polyclonal antibody (12258-1-AP)

    WB analysis of mouse testis using 12258-1-AP (same clone as 12258-1-PBS)

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 12258-1-AP (TBPL1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12258-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of mouse testis tissue using TBPL1 Polyclonal antibody (12258-1-AP)

    IP experiment of mouse testis using 12258-1-AP (same clone as 12258-1-PBS)

    IP result of anti-TBPL1 (IP:12258-1-AP, 4ug; Detection:12258-1-AP 1:300) with mouse testis tissue lysate 3920 ug. This data was developed using the same antibody clone with 12258-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using TBPL1 Polyclonal antibody (12258-1-AP)

    IHC staining of human stomach cancer using 12258-1-AP (same clone as 12258-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 12258-1-AP (TBPL1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12258-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using TBPL1 Polyclonal antibody (12258-1-AP)

    IHC staining of human stomach cancer using 12258-1-AP (same clone as 12258-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 12258-1-AP (TBPL1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12258-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse stomach tissue using TBPL1 Polyclonal antibody (12258-1-AP)

    IHC staining of mouse stomach using 12258-1-AP (same clone as 12258-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 12258-1-AP (TBPL1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12258-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse stomach tissue using TBPL1 Polyclonal antibody (12258-1-AP)

    IHC staining of mouse stomach using 12258-1-AP (same clone as 12258-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 12258-1-AP (TBPL1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12258-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat testis tissue using TBPL1 Polyclonal antibody (12258-1-AP)

    IHC staining of rat testis using 12258-1-AP (same clone as 12258-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 12258-1-AP (TBPL1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12258-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat testis tissue using TBPL1 Polyclonal antibody (12258-1-AP)

    IHC staining of rat testis using 12258-1-AP (same clone as 12258-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 12258-1-AP (TBPL1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12258-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using TBPL1 Polyclonal antibody (12258-1-AP)

    IHC staining of mouse testis using 12258-1-AP (same clone as 12258-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 12258-1-AP (TBPL1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12258-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using TBPL1 Polyclonal antibody (12258-1-AP)

    IHC staining of mouse testis using 12258-1-AP (same clone as 12258-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 12258-1-AP (TBPL1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12258-1-PBS in a different storage buffer formulation.

TBPL1 Polyclonal antibody, PBS Only

TBPL1 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 12258-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

12258-1-PBS targets TBPL1 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag2880

Product name: Recombinant human TBPL1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-186 aa of BC017559

Sequence: MDADSDVALDILITNVVCVFRTRCHLNLRKIALEGANVIYKRDVGKVLMKLRKPRITATIWSSGKIICTGATSEEEAKFGARRLARSLQKLGFQVIFTDFKVVNVLAVCNMPFEIRLPEFTKNNRPHASYEPELHPAVCYRIKSLRATLQIFSTGSITVTGPNVKAVATAVEQIYPFVFESRKEIL

相同性解析による交差性が予測される生物種
Full Name TBP-like 1
Calculated molecular weight 186 aa, 21 kDa
Observed molecular weight 21 kDa, 25 kDa
GenBank accession numberBC017559
Gene Symbol TBPL1
Gene ID (NCBI) 9519
RRIDAB_10642841
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP62380
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.
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