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Validation Data Gallery

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  • Western Blot (WB) analysis of MCF-7 cells using TBL2 Monoclonal antibody (66307-1-Ig)

    WB analysis of MCF-7 using 66307-1-Ig (same clone as 66307-1-PBS)

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 66307-1-Ig (TBL2 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66307-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Jurkat cells using TBL2 Monoclonal antibody (66307-1-Ig)

    WB analysis of Jurkat using 66307-1-Ig (same clone as 66307-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 66307-1-Ig (TBL2 Antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66307-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of COLO 320 cells using TBL2 Monoclonal antibody (66307-1-Ig)

    WB analysis of COLO 320 using 66307-1-Ig (same clone as 66307-1-PBS)

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 66307-1-Ig (TBL2 Antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66307-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using TBL2 Monoclonal antibody (66307-1-Ig)

    IHC staining of human liver using 66307-1-Ig (same clone as 66307-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66307-1-Ig (TBL2 Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 66307-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using TBL2 Monoclonal antibody (66307-1-Ig)

    IHC staining of human colon using 66307-1-Ig (same clone as 66307-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66307-1-Ig (TBL2 Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 66307-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using TBL2 Monoclonal antibody (66307-1-Ig)

    IHC staining of human colon using 66307-1-Ig (same clone as 66307-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66307-1-Ig (TBL2 Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 66307-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using TBL2 Monoclonal antibody (66307-1-Ig)

    IHC staining of human liver using 66307-1-Ig (same clone as 66307-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66307-1-Ig (TBL2 Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 66307-1-PBS in a different storage buffer formulation.

TBL2 Monoclonal antibody, PBS Only

TBL2 Monoclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 66307-1-PBS
Clone No.1D5C2

Host / Isotype

Mouse / IgA

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Synonyms

TBL2, transducin (beta) like 2, Transducin beta like protein 2, WBSCR13, WS betaTRP

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Mouse monoclonal antibodies of IgA isotype can be detected with "anti-mouse IgG (H+L)" secondary antibodies.

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66307-1-PBS targets TBL2 in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgA
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag24225

Product name: Recombinant human TBL2 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 100-446 aa of BC012938

Sequence: SNGKYLATCADDRTIRIWSTKDFLQREHRSMRANVELDHATLVRFSPDCRAFIVWLANGDTLRVFKMTKREDGGYTFTATPEDFPKKHKAPVIDIGIANTGKFIMTASSDTTVLIWSLKGQVLSTINTNQMNNTHAAVSPCGRFVASCGFTPDVKVWEVCFGKKGEFQEVVRAFELKGHSAAVHSFAFSNDSRRMASVSKDGTWKLWDTDVEYKKKQDPYLLKTGRFEEAAGAAPCRLALSPNAQVLALASGSSIHLYNTRRGEKEECFERVHGECIANLSFDITGRFLASCGDRAVRLFHNTPGHRAMVEEMQGHLKRASNESTRQRLQQQLTQAQVTLGGPGAED

相同性解析による交差性が予測される生物種
Full Name transducin (beta)-like 2
Calculated molecular weight 446 aa, 50 kDa
Observed molecular weight 50 kDa
GenBank accession numberBC012938
Gene Symbol TBL2
Gene ID (NCBI) 26608
RRIDAB_2881689
Conjugate Unconjugated
Form
FormLiquid
Purification MethodThiophilic affinity chromatograph
UNIPROT IDQ9Y4P3
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

TBL2 belongs to a member of the beta-transducin protein family. This gene is commonly deleted in Williams-Beuren syndrome, a developmental disorder with multi-system manifestations caused by haploinsufficiency for contiguous genes deleted in chromosome region 7q11.23. TBL2 encodes a protein with four putative WD40 repeats, which may be involved in some intracellular signaling pathway.

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