Validation Data Gallery

Filter: AllWBIPIHCIF/ICC

WB analysis of COLO 320 using 12488-1-AP
COLO 320 cells were subjected to SDS PAGE followed by western blot with 12488-1-AP (TBL2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 12488-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 12488-1-AP (TBL2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
WB analysis of COLO 320 using 12488-1-AP
COLO 320 cells were subjected to SDS PAGE followed by western blot with 12488-1-AP (TBL2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
WB analysis of COLO 320 using 12488-1-AP
COLO 320 cells were subjected to SDS PAGE followed by western blot with 12488-1-AP (TBL2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of COLO 320 using 12488-1-AP
COLO 320 cells were subjected to SDS PAGE followed by western blot with 12488-1-AP (TBL2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse pancreas using 12488-1-AP
mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 12488-1-AP (TBL2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse placenta using 12488-1-AP
mouse placenta tissue were subjected to SDS PAGE followed by western blot with 12488-1-AP (TBL2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of Jurkat using 12488-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 12488-1-AP (TBL2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
WB analysis of human placenta using 12488-1-AP
human placenta tissue were subjected to SDS PAGE followed by western blot with 12488-1-AP (TBL2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IP experiment of COLO 320 using 12488-1-AP
IP result of anti-TBL2 (IP:12488-1-AP, 4ug; Detection:12488-1-AP 1:800) with COLO 320 cells lysate 2400ug.
IHC staining of human pancreas cancer using 12488-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 12488-1-AP (TBL2 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human pancreas using 12488-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas using 12488-1-AP (TBL2 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human pancreas using 12488-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas using 12488-1-AP (TBL2 antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human heart using 12488-1-AP
Immunohistochemical analysis of paraffin-embedded human heart using 12488-1-AP (TBL2 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human heart using 12488-1-AP
Immunohistochemical analysis of paraffin-embedded human heart using 12488-1-AP (TBL2 antibody) at dilution of 1:50 (under 40x lens).
IF Staining of HepG2 using 12488-1-AP
Immunofluorescent analysis of () fixed HepG2 cells using 12488-1-AP (TBL2 antibody) at dilution of 1:25.

Featured Product
KD/KO Validated

TBL2 Polyclonal antibody

TBL2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 12488-1-AP

Publications(6)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

Williams-Beuren syndrome chromosomal region 13 protein, WBSCR13, UNQ563/PRO1125, Transducin beta-like protein 2, Transducin beta like protein 2

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (6)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	COLO 320 cells, HeLa cells, human placenta tissue, Jurkat cells, mouse pancreas tissue, mouse placenta tissue
Positive IP detected in	COLO 320 cells
Positive IHC detected in	human pancreas cancer tissue, human heart tissue, human pancreas tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:20-1:200
Immunofluorescence (IF)/ICC	IF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 2 publications below
WB	See 6 publications below
IHC	See 2 publications below
IF	See 3 publications below

Product Information

12488-1-AP targets TBL2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag3200 Product name: Recombinant human TBL2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 100-446 aa of BC012938 Sequence: SNGKYLATCADDRTIRIWSTKDFLQREHRSMRANVELDHATLVRFSPDCRAFIVWLANGDTLRVFKMTKREDGGYTFTATPEDFPKKHKAPVIDIGIANTGKFIMTASSDTTVLIWSLKGQVLSTINTNQMNNTHAAVSPCGRFVASCGFTPDVKVWEVCFGKKGEFQEVVRAFELKGHSAAVHSFAFSNDSRRMASVSKDGTWKLWDTDVEYKKKQDPYLLKTGRFEEAAGAAPCRLALSPNAQVLALASGSSIHLYNTRRGEKEECFERVHGECIANLSFDITGRFLASCGDRAVRLFHNTPGHRAMVEEMQGHLKRASNESTRQRLQQQLTQAQVTLGGPGAED 相同性解析による交差性が予測される生物種
Full Name	transducin (beta)-like 2
Calculated molecular weight	446 aa, 50 kDa
Observed molecular weight	45-50 kDa
GenBank accession number	BC012938
Gene Symbol	TBL2
Gene ID (NCBI)	26608
RRID	AB_2199923
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9Y4P3
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

TBL2 belongs to a member of the beta-transducin protein family. This gene is commonly deleted in Williams-Beuren syndrome, a developmental disorder with multi-system manifestations caused by haploinsufficiency for contiguous genes deleted in chromosome region 7q11.23. TBL2 encodes a protein with four putative WD40 repeats, which may be involved in some intracellular signaling pathway.

Protocols

Product Specific Protocols
IF protocol for TBL2 antibody 12488-1-AP	Download protocol
IHC protocol for TBL2 antibody 12488-1-AP	Download protocol
IP protocol for TBL2 antibody 12488-1-AP	Download protocol
WB protocol for TBL2 antibody 12488-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB,IHC	Adv Sci (Weinh) TBL2 Promotes Tumorigenesis via PRMT5/WDR77-Mediated AKT Activation in Breast Cancer Authors - Xiuqing Lu View Article
	WB,IF	Adv Sci (Weinh) Primate-Specific DAZ Regulates Translation of Cell Proliferation-Related mRNAs and is Essential for Maintenance of Spermatogonia Authors - Ningjing Ou View Article
human	WB,IF	PLoS One TBL2 Is a Novel PERK-Binding Protein that Modulates Stress-Signaling and Cell Survival during Endoplasmic Reticulum Stress. Authors - Yoshinori Tsukumo KD Validated View Article
	WB	J Cell Biochem TBL2 Associates With ATF4 mRNA Via Its WD40 Domain and Regulates Its Translation During ER Stress. Authors - Yoshinori Tsukumo View Article
human	WB, IF	Biochem Biophys Res Commun The endoplasmic reticulum-localized protein TBL2 interacts with the 60S ribosomal subunit. Authors - Yoshinori Tsukumo View Article
human	WB,IHC	Ann Surg Oncol Transducin Beta-Like 2 is a Potential Driver Gene that Adapts to Endoplasmic Reticulum Stress to Promote Tumor Growth of Lung Adenocarcinoma Authors - Keisuke Kosai KD Validated View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of COLO 320 using 12488-1-AP

WB analysis of HeLa using 12488-1-AP

WB analysis of COLO 320 using 12488-1-AP

WB analysis of COLO 320 using 12488-1-AP

WB analysis of COLO 320 using 12488-1-AP

WB analysis of mouse pancreas using 12488-1-AP

WB analysis of mouse placenta using 12488-1-AP

WB analysis of Jurkat using 12488-1-AP

WB analysis of human placenta using 12488-1-AP

IP experiment of COLO 320 using 12488-1-AP

IHC staining of human pancreas cancer using 12488-1-AP

IHC staining of human pancreas using 12488-1-AP

IHC staining of human pancreas using 12488-1-AP

IHC staining of human heart using 12488-1-AP

IHC staining of human heart using 12488-1-AP

IF Staining of HepG2 using 12488-1-AP

TBL2 Polyclonal antibody

TBL2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

TBL2 Promotes Tumorigenesis via PRMT5/WDR77-Mediated AKT Activation in Breast Cancer

Primate-Specific DAZ Regulates Translation of Cell Proliferation-Related mRNAs and is Essential for Maintenance of Spermatogonia

TBL2 Is a Novel PERK-Binding Protein that Modulates Stress-Signaling and Cell Survival during Endoplasmic Reticulum Stress.

TBL2 Associates With ATF4 mRNA Via Its WD40 Domain and Regulates Its Translation During ER Stress.

The endoplasmic reticulum-localized protein TBL2 interacts with the 60S ribosomal subunit.

Transducin Beta-Like 2 is a Potential Driver Gene that Adapts to Endoplasmic Reticulum Stress to Promote Tumor Growth of Lung Adenocarcinoma