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Species Reactivity

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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TBC1D9B Polyclonal antibody (20177-1-AP)

    WB analysis using 20177-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 20177-1-AP (TBC1D9B antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of Jurkat cells using TBC1D9B Polyclonal antibody (20177-1-AP)

    IP experiment of Jurkat using 20177-1-AP

    IP result of anti-TBC1D9B (IP:20177-1-AP, 4ug; Detection:20177-1-AP 1:1000) with Jurkat cells lysate 1920 ug.

  • Immunoprecipitation (IP) experiment of HEK-293 cells using TBC1D9B Polyclonal antibody (20177-1-AP)

    IP experiment of HEK-293 using 20177-1-AP

    IP result of anti-TBC1D9B (IP:20177-1-AP, 4ug; Detection:20177-1-AP 1:1000) with HEK-293 cells lysate 1800 ug.

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TBC1D9B Polyclonal antibody

TBC1D9B Polyclonal Antibody for WB, IP, ELISA
Cat No. 20177-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, ELISA

Synonyms

TBC1 domain family member 9B

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells, Jurkat cells
Positive IP detected inJurkat cells, HEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

20177-1-AP targets TBC1D9B in WB, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TBC1D9B fusion protein Ag14019 相同性解析による交差性が予測される生物種
Full Name TBC1 domain family, member 9B (with GRAM domain)
Calculated molecular weight 1250 aa, 140 kDa
Observed molecular weight 141-150 kDa
GenBank accession numberBC000701
Gene Symbol TBC1D9B
Gene ID (NCBI) 23061
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ66K14
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TBC1 domain family member 9B (TBC1D9B) a Rab11A GTPase-activating protein (Rab11A GAP), as a LMTK1 binding protein, and TBC1D9B inactivates Rab11A under the control of LMTK1A (PMID: 31628178). TBC1D9B is associated with endocytic vesicles, including those likely to contain active Rab11a (PMID: 25232007). TBC1D9B could also interact with other mammalian ATG8 homologues. TBC1D9B could be co-localized with LC3B on the autophagosome membranes (PMID: 30202024).

Protocols

Product Specific Protocols
WB protocol for TBC1D9B antibody 20177-1-APDownload protocol
IP protocol for TBC1D9B antibody 20177-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols