Validation Data Gallery
Tested Applications
| Positive WB detected in | HEK-293 cells, HeLa cells, Jurkat cells |
| Positive IP detected in | Jurkat cells, HEK-293 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:12000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
20177-1-AP targets TBC1D9B in WB, IP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag14019 Product name: Recombinant human TBC1D9B protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1069-1250 aa of BC000701 Sequence: ARTGRKPRDCATEEDEPPAPELHQDAARELQPPAAGDPQAKAGGDTHLGTAPQESQVVVEGGSGEGQGSPSQLLSDDETKDDMSMSSYSVVSTGSLQCEDLADDTVLVGGEACSPTARIGGTVDTDWCISFEQILASILTESVLVNFFEKRVDIGLKIKDQKKVERQFSTASDHEQPGVSG 相同性解析による交差性が予測される生物種 |
| Full Name | TBC1 domain family, member 9B (with GRAM domain) |
| Calculated molecular weight | 1250 aa, 140 kDa |
| Observed molecular weight | 141-150 kDa |
| GenBank accession number | BC000701 |
| Gene Symbol | TBC1D9B |
| Gene ID (NCBI) | 23061 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q66K14 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
TBC1 domain family member 9B (TBC1D9B) a Rab11A GTPase-activating protein (Rab11A GAP), as a LMTK1 binding protein, and TBC1D9B inactivates Rab11A under the control of LMTK1A (PMID: 31628178). TBC1D9B is associated with endocytic vesicles, including those likely to contain active Rab11a (PMID: 25232007). TBC1D9B could also interact with other mammalian ATG8 homologues. TBC1D9B could be co-localized with LC3B on the autophagosome membranes (PMID: 30202024).
