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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TBC1D4 Polyclonal antibody (20024-1-AP)

    WB analysis using 20024-1-AP (same clone as 20024-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 20024-1-AP (TBC1D4 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20024-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse colon tissue using TBC1D4 Polyclonal antibody (20024-1-AP)

    IHC staining of mouse colon using 20024-1-AP (same clone as 20024-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 20024-1-AP (TBC1D4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20024-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse colon tissue using TBC1D4 Polyclonal antibody (20024-1-AP)

    IHC staining of mouse colon using 20024-1-AP (same clone as 20024-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 20024-1-AP (TBC1D4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20024-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat skeletal muscle tissue using TBC1D4 Polyclonal antibody (20024-1-AP)

    IHC staining of rat skeletal muscle using 20024-1-AP (same clone as 20024-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue slide using 20024-1-AP (TBC1D4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20024-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using TBC1D4 Polyclonal antibody (20024-1-AP)

    IHC staining of mouse skeletal muscle using 20024-1-AP (same clone as 20024-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 20024-1-AP (TBC1D4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20024-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using TBC1D4 Polyclonal antibody (20024-1-AP)

    IF Staining of HeLa using 20024-1-AP (same clone as 20024-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using TBC1D4 antibody (20024-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 20024-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HeLa cells using TBC1D4 Polyclonal antibody (20024-1-AP)

    FC experiment of HeLa using 20024-1-AP (same clone as 20024-1-PBS)

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human TBC1D4 (20024-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP, Clone: ) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 20024-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of Jurkat cells using TBC1D4 Polyclonal antibody (20024-1-AP)

    FC experiment of Jurkat using 20024-1-AP (same clone as 20024-1-PBS)

    1X10^6 Jurkat cells were intracellularly stained with 0.4 ug Anti-Human TBC1D4 (20024-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP, Clone: ) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 20024-1-PBS in a different storage buffer formulation.

TBC1D4 Polyclonal antibody, PBS Only

TBC1D4 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 20024-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

20024-1-PBS targets TBC1D4 in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

Peptide

相同性解析による交差性が予測される生物種
Full Name TBC1 domain family, member 4
Calculated molecular weight 147 kDa
Observed molecular weight 160 kDa
GenBank accession numberNM_014832
Gene Symbol TBC1D4
Gene ID (NCBI) 9882
RRIDAB_3085594
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDO60343
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

TBC1D4, also named as AS160, KIAA0603, may act as a GTPase-activating protein for RAB2A, RAB8A, RAB10 and RAB14. Isoform 2 promotes insulin-induced glucose transporter SLC2A4/GLUT4 translocation at the plasma membrane, thus increasing glucose uptake. The antibody recognizes all the isoform of TBC1D4.

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