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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

TBC1D24 Polyclonal antibody

TBC1D24 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 25254-1-AP

Synonyms

KIAA1171, TBC1 domain family member 24, TBC1 domain family, member 24, TBC1D24

Validation Data Gallery

Applications:
  • WB analysis using 25254-1-AP

    WB analysis using 25254-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 25254-1-AP (TBC1D24 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • IHC staining of human stomach using 25254-1-AP

    IHC staining of human stomach using 25254-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 25254-1-AP (TBC1D24 Antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human stomach using 25254-1-AP

    IHC staining of human stomach using 25254-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 25254-1-AP (TBC1D24 Antibody) at dilution of 1:200 (under 40x lens).

  • IHC staining of human kidney using 25254-1-AP

    IHC staining of human kidney using 25254-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 25254-1-AP (TBC1D24 Antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human kidney using 25254-1-AP

    IHC staining of human kidney using 25254-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 25254-1-AP (TBC1D24 Antibody) at dilution of 1:200 (under 40x lens).

Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells
Positive IHC detected inhuman stomach tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

25254-1-AP targets TBC1D24 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TBC1D24 fusion protein Ag19380 相同性解析による交差性が予測される生物種
Full Name TBC1 domain family, member 24
Calculated molecular weight 559 aa, 63 kDa
Observed molecular weight 60-63 kDa
GenBank accession numberBC127015
Gene symbol TBC1D24
Gene ID (NCBI) 57465
RRIDAB_2879990
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for TBC1D24 antibody 25254-1-APDownload protocol
IHC protocol for TBC1D24 antibody 25254-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Cell Mol Life Sci

The Ncoa7 locus regulates V-ATPase formation and function, neurodevelopment and behaviour.

Authors - Enrico Castroflorio
View Article
human,mouseIHC, WB

PLoS One

Gm14230 controls Tbc1d24 cytoophidia and neuronal cellular juvenescence.

Authors - Takao Morimune
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Xin (Verified Customer) (03-16-2022)

A good antibody to detect TBC1D24

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:2000
  • Cell Tissue Type: human postmortem brain lysate
TBC1D24 Antibody Western Blot validation (1:2000 dilution) in human postmortem brain lysate (Cat no:25254-1-AP)