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Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using TBC1D22A Polyclonal antibody (18332-1-AP)

    WB analysis of HeLa using 18332-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 18332-1-AP (TBC1D22A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using TBC1D22A Polyclonal antibody (18332-1-AP)

    WB analysis of HeLa using 18332-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 18332-1-AP (TBC1D22A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using TBC1D22A Polyclonal antibody (18332-1-AP)

    WB analysis of HeLa using 18332-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 18332-1-AP (TBC1D22A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of COLO 320 cells using TBC1D22A Polyclonal antibody (18332-1-AP)

    WB analysis of COLO 320 using 18332-1-AP

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 18332-1-AP (TBC1D22A antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of COLO 320 cells using TBC1D22A Polyclonal antibody (18332-1-AP)

    WB analysis of COLO 320 using 18332-1-AP

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 18332-1-AP (TBC1D22A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using TBC1D22A Polyclonal antibody (18332-1-AP)

    IHC staining of human cervical cancer using 18332-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 18332-1-AP (TBC1D22A Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using TBC1D22A Polyclonal antibody (18332-1-AP)

    IHC staining of human cervical cancer using 18332-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 18332-1-AP (TBC1D22A Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using TBC1D22A Polyclonal antibody (18332-1-AP)

    IHC staining of human kidney using 18332-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 18332-1-AP (TBC1D22A Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using TBC1D22A Polyclonal antibody (18332-1-AP)

    IHC staining of human kidney using 18332-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 18332-1-AP (TBC1D22A Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human cervix tissue using TBC1D22A Polyclonal antibody (18332-1-AP)

    IHC staining of human cervix using 18332-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervix using 18332-1-AP (TBC1D22A antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human cervix tissue using TBC1D22A Polyclonal antibody (18332-1-AP)

    IHC staining of human cervix using 18332-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervix using 18332-1-AP (TBC1D22A antibody) at dilution of 1:100 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using TBC1D22A Polyclonal antibody (18332-1-AP)

    IF Staining of HeLa using 18332-1-AP

    Immunofluorescent analysis of HeLa cells using 18332-1-AP (TBC1D22A antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

TBC1D22A Polyclonal antibody

TBC1D22A Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 18332-1-AP
Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

TBC1 domain family, member 22A, TBC1 domain family member 22A, HSC79E021, C22orf4

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Positive WB detected inHeLa cells, COLO 320 cells
Positive IHC detected inhuman cervical cancer tissue, human cervix tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

18332-1-AP targets TBC1D22A in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag12942

Product name: Recombinant human TBC1D22A protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-517 aa of BC001292

Sequence: MASDGARKQFWKRSNSKLPGSIQHVYGAQHPPFDPLLHGTLLRSTAKMPTTPVKAKRVSTFQEFESNTSDAWDAGEDDDELLAMAAESLNSKVVMETANRVLRNHSQRQGRPTLQEGPGLQQKPRPEAEPPSPPSGDLRLVKSVSESHTSCPAESASDAAPLQRSQSLPHSATVTLGGTSDPSTLSSSALSEREASRLDKFKQLLAGPNTDLEELRRLSWSGIPKPVRPMTWKLLSGYLPANVDRRPATLQRKQKEYFAFIEHYYDSRNDEVHQDTYRQIHIDIPRMSPEALILQPKVTEIFERILFIWAIRHPASGYVQGINDLVTPFFVVFICEYIEAEEVDTVDVSCVPAEVLCNIEADTYWCMSKLLDGIQDNYTFAQPGIQMKVKMLEELVSRIDEQVHRHLDQHEVRYLQFAFRWMNNLLMREVPLRCTIRLWDTYQSEPDGFSHFHLYVCAAFLVRWRKEILEEKDFQELLLFLQNLPTAHWDDEDISLLLAEAYRLKFAFADAPNHYKK

相同性解析による交差性が予測される生物種
Full Name TBC1 domain family, member 22A
Calculated molecular weight 59 kDa
Observed molecular weight 59 kDa
GenBank accession numberBC001292
Gene Symbol TBC1D22A
Gene ID (NCBI) 25771
RRIDAB_2302953
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8WUA7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for TBC1D22A antibody 18332-1-APDownload protocol
IHC protocol for TBC1D22A antibody 18332-1-APDownload protocol
IF protocol for TBC1D22A antibody 18332-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC

Int J Med Sci

High Expression of TBC1 Domain Family Member 22A is Related to Poor Prognosis in Ovarian Serous Cystadenocarcinoma

Authors - Xiaofeng Lv
View Article