Validation Data Gallery
Tested Applications
| Positive WB detected in | A549 cells, pig brain tissue, LNCaP cells, HeLa cells, Jurkat cells, A172 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
60975-3-Ig targets TBC1D17 in WB, ELISA applications and shows reactivity with human, pig, rabbit samples.
| Tested Reactivity | human, pig, rabbit |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag14358 Product name: Recombinant human TBC1D17 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-350 aa of BC003516 Sequence: MEGAGYRVVFEKGGVYLHTSAKKYQDRDSLIAGVIRVVEKDNDVLLHWAPVEEAGDSTQILFSKKDSSGGDSCASEEEPTFDPGYEPDWAVISTVRPQPCHSEPTRGAEPSCPQGSWAFSVSLGELKSIRRSKPGLSWAYLVLVTQAGGSLPALHFHRGGTRALLRVLSRYLLLASSPQDSRLYLVFPHDSSALSNSFHHLQLFDQDSSNVVSRFLQDPYSTTFSSFSRVTNFFRGALQPQPEGAASDLPPPPDDEPEPGFEVISCVELGPRPTVERGPPVTEEEWARHVGPEGRLQQVPELKNRIFSGGLSPSLRREAWKFLLGYLSWEGTAEEHKAHIRKKTDEYFRM 相同性解析による交差性が予測される生物種 |
| Full Name | TBC1 domain family, member 17 |
| Calculated molecular weight | 648 aa, 73 kDa |
| Observed molecular weight | 73 kDa |
| GenBank accession number | BC003516 |
| Gene Symbol | TBC1D17 |
| Gene ID (NCBI) | 79735 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q9HA65 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
TBC1 domain family member 17 (TBC1D17), a Rab GTPase-activating protein, through its catalytic activity, regulates Rab8-mediated endocytic trafficking of transferrin receptor (PMID: 22854040). TBC1D17 also inhibits the trafficking of Shiga toxin from plasma membrane to the Golgi complex through its catalytic activity (PMID: 1756278).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for TBC1D17 antibody 60975-3-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |