Validation Data Gallery
Tested Applications
| Positive WB detected in | HL-60 cells, mouse spleen tissue, rat spleen tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
34255-1-AP targets TBC1D10C in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag40608 Product name: Recombinant human TBC1D10C protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 26-138 aa of BC062999 Sequence: SELSGPGPYRQADRYGFIGGSSAEPGPGHPPADLIRQREMKWVEMTSHWEKTMSRRYKKVKMQCRKGIPSALRARCWPLLCGAHVCQKNSPGTYQELAEAPGDPQWMETIGRD 相同性解析による交差性が予測される生物種 |
| Full Name | TBC1 domain family, member 10C |
| Calculated molecular weight | 446 aa, 50 kDa |
| Observed molecular weight | 47 kDa |
| GenBank accession number | BC062999 |
| Gene Symbol | TBC1D10C |
| Gene ID (NCBI) | 374403 |
| RRID | AB_3743181 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity Purification |
| UNIPROT ID | Q8IV04 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
TBC1D10C (also known as Carabin or EPI64C) is a negative regulator of dual signaling pathways. It can inhibit the activity of small GTPase Ras through its N-terminal TBC domain, and also suppress the activity of calcineurin through its C-terminal binding site, thereby simultaneously negatively regulating the two key signaling pathways, Ras-MAPK and Ca²⁺-Calcineurin-NFAT.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for TBC1D10C antibody 34255-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |