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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TAU Recombinant monoclonal antibody (82846-5-RR)

    WB analysis using 82846-5-RR (same clone as 82846-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82846-5-RR (TAU antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82846-5-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using TAU Recombinant monoclonal antibody (82846-5-RR)

    IHC staining of human colon using 82846-5-RR (same clone as 82846-5-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 82846-5-RR (TAU antibody) at dilution of 1:3000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82846-5-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using TAU Recombinant monoclonal antibody (82846-5-RR)

    IHC staining of human colon using 82846-5-RR (same clone as 82846-5-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 82846-5-RR (TAU antibody) at dilution of 1:3000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82846-5-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using TAU Recombinant monoclonal antibody (82846-5-RR)

    IF Staining of mouse brain using 82846-5-RR (same clone as 82846-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using TAU antibody (82846-5-RR, Clone: 252452C8 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82846-5-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using TAU Recombinant monoclonal antibody (82846-5-RR)

    IF Staining of mouse brain using 82846-5-RR (same clone as 82846-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using TAU antibody (82846-5-RR, Clone: 252452C8 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82846-5-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using TAU Recombinant monoclonal antibody (82846-5-RR)

    IF Staining of mouse brain using 82846-5-RR (same clone as 82846-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse brain tissue using TAU antibody (82846-5-RR, Clone: 252452C8 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 82846-5-PBS in a different storage buffer formulation.

  • Affinity of 82846-5-RR

    Affinity of 82846-5-RR

    Biolayer interferometry (BLI) kinetic assays of 82846-5-RR against Human TAU were performed. The affinity constant is below 1 pM.

TAU Recombinant monoclonal antibody, PBS Only

TAU Uni-rAb® Recombinant Antibody for WB, IHC, IF-P, Indirect ELISA
Cat No. 82846-5-PBS
Clone No.252452C8

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, Indirect ELISA

Synonyms

MAPT, AT180, DDPAC, MAPTL, Microtubule-associated protein tau

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

82846-5-PBS targets TAU in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Eg6232

Product name: Recombinant human TAU protein

Source: mammalian cells-derived, V37

Tag: C-rFc

Domain: 1-758 aa of NM_016835.4

Sequence: MAEPRQEFEVMEDHAGTYGLGDRKDQGGYTMHQDQEGDTDAGLKESPLQTPTEDGSEEPGSETSDAKSTPTAEDVTAPLVDEGAPGKQAAAQPHTEIPEGTTAEEAGIGDTPSLEDEAAGHVTQEPESGKVVQEGFLREPGPPGLSHQLMSGMPGAPLLPEGPREATRQPSGTGPEDTEGGRHAPELLKHQLLGDLHQEGPPLKGAGGKERPGSKEEVDEDRDVDESSPQDSPPSKASPAQDGRPPQTAAREATSIPGFPAEGAIPLPVDFLSKVSTEIPASEPDGPSVGRAKGQDAPLEFTFHVEITPNVQKEQAHSEEHLGRAAFPGAPGEGPEARGPSLGEDTKEADLPEPSEKQPAAAPRGKPVSRVPQLKARMVSKSKDGTGSDDKKAKTSTRSSAKTLKNRPCLSPKHPTPGSSDPLIQPSSPAVCPEPPSSPKYVSSVTSRTGSSGAKEMKLKGADGKTKIATPRGAAPPGQKGQANATRIPAKTPPAPKTPPSSGEPPKSGDRSGYSSPGSPGTPGSRSRTPSLPTPPTREPKKVAVVRTPPKSPSSAKSRLQTAPVPMPDLKNVKSKIGSTENLKHQPGGGKVQIINKKLDLSNVQSKCGSKDNIKHVPGGGSVQIVYKPVDLSKVTSKCGSLGNIHHKPGGGQVEVKSEKLDFKDRVQSKIGSLDNITHVPGGGNKKIETHKLTFRENAKAKTDHGAEIVYKSPVVSGDTSPRHLSNVSSTGSIDMVDSPQLATLADEVSASLAKQGL

相同性解析による交差性が予測される生物種
Full Name microtubule-associated protein tau
Calculated molecular weight79 kDa
Observed molecular weight 45-70 kDa
GenBank accession numberNM_016835.4
Gene Symbol TAU
Gene ID (NCBI) 4137
RRIDAB_3743441
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP10636
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Tau (tubulin-associated unit) is a microtubule-associated protein (also known as MAPT), expressed mainly in neurons of the central nervous system. Its primary function is to modulate microtubule dynamics for maintaining axonal cytoskeleton. Various isoforms of Tau exist due to the alternative splicing, and short isoforms around 45-69 kDa and long isoforms around 100-110 kDa have been reported in different literature (8752131,15965697).

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