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TAU Monoclonal antibody

TAU Monoclonal Antibody for IHC, WB, ELISA

Host / Isotype

Mouse / IgG2c

Reactivity

Human, mouse, rat, pig and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

CloneNo.

1E9A8

Cat no : 66499-1-Ig

Synonyms

DDPAC, FTDP 17, MAPT, MAPTL, MSTD, MTBT1, MTBT2, Neurofibrillary tangle protein, Paired helical filament tau, PHF tau, PPND, TAU



Tested Applications

Positive WB detected inHeLa cells, Y79 cells, pig brain tissue, SH-SY5Y cells, U-251 cells, Neuro-2a cells, rat brain tissue, mouse brain tissue
Positive IHC detected inhuman gliomas tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:30000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Sample-dependent, check data in validation data gallery

Product Information

66499-1-Ig targets TAU in WB, IHC, IF, ELISA applications and shows reactivity with Human, mouse, rat, pig samples.

Tested Reactivity Human, mouse, rat, pig
Cited Reactivity human, rat, mouse
Host / Isotype Mouse / IgG2c
Class Monoclonal
Type Antibody
Immunogen TAU fusion protein Ag21926 相同性解析による交差性が予測される生物種
Full Name microtubule-associated protein tau
Calculated molecular weight 37-46, 79-81 kDa
Observed molecular weight 100 kDa
GenBank accession numberBC000558
Gene symbol MAPT
Gene ID (NCBI) 4137
RRIDAB_2881863
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The microtubule-associated protein TAU (MAPT or TAU) is encoded by MAPT gene, which locates on human chromosome 17q21, binds to the tubulin subunit of microtubule and promotes its assembly and stability. Most TAU is expressed in neurons, and TAU isoform is expressed in the peripheral nervous system while the others are expressed in the central nervous system. TAU links axonal microtubules with C-terminus to neural plasma membrane components with its N-terminus, suggesting the participation in intracellular signal transduction and neuron's development and viability. Various isoforms of Tau exist due to the alternative splicing, and short isoforms around 45-69 kDa and long isoforms around 100-110 kDa have been reported in different literature (PMID:8752131,15965697, 12485403).Present monoclonal anti-Tau antibody can detect approx 100-kDa bands in brain tissues.

Protocols

Product Specific Protocols
WB protocol for TAU antibody 66499-1-IgDownload protocol
IHC protocol for TAU antibody 66499-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Acta Pharmacol Sin

Long-term running exercise alleviates cognitive dysfunction in APP/PSEN1 transgenic mice via enhancing brain lysosomal function.

Authors - Xue Wang
humanWB

Int J Mol Sci

Neuroprotection and Mechanism of Gas-miR36-5p from Gastrodia elata in an Alzheimer's Disease Model by Regulating Glycogen Synthase Kinase-3β

Authors - Zhongteng Lu
mouseWB

Front Aging Neurosci

Salidroside Ameliorates Alzheimer's Disease by Targeting NLRP3 Inflammasome-Mediated Pyroptosis.

Authors - Yawen Cai
mouseWB

Mol Med

Eriodictyol ameliorates cognitive dysfunction in APP/PS1 mice by inhibiting ferroptosis via vitamin D receptor-mediated Nrf2 activation.

Authors - Lin Li
mouseWB

Environ Sci Pollut Res Int

Acute lead acetate induces neurotoxicity through decreased synaptic plasticity-related protein expression and disordered dendritic formation in nerve cells.

Authors - Lingli Chen
mouseWB

Comput Math Methods Med

Acupuncture Effect Assessment in APP/PS1 Transgenic Mice: On Regulating Learning-Memory Abilities, Gut Microbiota, and Microbial Metabolites.

Authors - Bo Yang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Virender (Verified Customer) (01-09-2020)

Tau antibody works well for recombinant tau as well.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:5000
FH

Mary (Verified Customer) (11-08-2019)

I like this antibody. It provides a clear specific signal. The shipping was prompt and the antibody was kept on ice the whole time which I appreciated.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: SH-SY5Y