Validation Data Gallery
Tested Applications
| Positive IHC detected in | mouse liver tissue, rat liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | A549 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
33698-1-AP targets TAT in IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag39227 Product name: Recombinant human TAT protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 294-454 aa of BC130534 Sequence: IHDRRDIFGNEIRDGLVKLSQRILGPCTIVQGALKSILCRTPGEFYHNTLSFLKSNADLCYGALAAIPGLRPVRPSGAMYLMVGIEMEHFPEFENDVEFTERLVAEQSVHCLPATCFEYPNFIRVVITVPEVMMLEACSRIQEFCEQHYHCAEGSQEECDK 相同性解析による交差性が予測される生物種 |
| Full Name | tyrosine aminotransferase |
| GenBank accession number | BC130534 |
| Gene Symbol | TAT |
| Gene ID (NCBI) | 6898 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity Purification |
| UNIPROT ID | P17735 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Tyrosine aminotransferase, also known as tyrosine transaminase, is the first enzyme in the catabolic pathway of tyrosine. The gene of this transaminase is regulated by glucocorticoid hormones as well as via the cAMP pathway. TAT reversibly catalyses Tyr to 4-hydroxyphenylpyruvate (4-HPP), which is the substrate for pathways producing plastoquinone31, 32, tocopherols33, phenolic acid14, 28, and benzylisoquinoline alkaloid (BIA)34 in plants, although tyrosine is synthesised from 4-HPP by TAT in bacteria (28687763).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for TAT antibody 33698-1-AP | Download protocol |
| IHC protocol for TAT antibody 33698-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |