Validation Data Gallery

Filter: AllWBIHCIF/ICC

WB analysis of HEK-293 using 16739-1-AP (same clone as 16739-1-PBS)
HEK-293 cells were subjected to SDS PAGE followed by western blot with 16739-1-AP (TASP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16739-1-PBS in a different storage buffer formulation.
WB analysis using 16739-1-AP (same clone as 16739-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 16739-1-AP (TASP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16739-1-PBS in a different storage buffer formulation.
WB analysis of HeLa using 16739-1-AP (same clone as 16739-1-PBS)
HeLa cells were subjected to SDS PAGE followed by western blot with 16739-1-AP (TASP1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16739-1-PBS in a different storage buffer formulation.
WB analysis of HeLa using 16739-1-AP (same clone as 16739-1-PBS)
HeLa cells were subjected to SDS PAGE followed by western blot with 16739-1-AP (TASP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16739-1-PBS in a different storage buffer formulation.
IHC staining of human ovary using 16739-1-AP (same clone as 16739-1-PBS)
Immunohistochemical analysis of paraffin-embedded human ovary using 16739-1-AP (TASP1 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 16739-1-PBS in a different storage buffer formulation.
IHC staining of human ovary using 16739-1-AP (same clone as 16739-1-PBS)
Immunohistochemical analysis of paraffin-embedded human ovary using 16739-1-AP (TASP1 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 16739-1-PBS in a different storage buffer formulation.
IHC staining of human kidney using 16739-1-AP (same clone as 16739-1-PBS)
Immunohistochemical analysis of paraffin-embedded human kidney using 16739-1-AP (TASP1 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 16739-1-PBS in a different storage buffer formulation.
IHC staining of human kidney using 16739-1-AP (same clone as 16739-1-PBS)
Immunohistochemical analysis of paraffin-embedded human kidney using 16739-1-AP (TASP1 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 16739-1-PBS in a different storage buffer formulation.
IHC staining of human testis using 16739-1-AP (same clone as 16739-1-PBS)
Immunohistochemical analysis of paraffin-embedded human testis using 16739-1-AP (TASP1 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 16739-1-PBS in a different storage buffer formulation.
IHC staining of human testis using 16739-1-AP (same clone as 16739-1-PBS)
Immunohistochemical analysis of paraffin-embedded human testis using 16739-1-AP (TASP1 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 16739-1-PBS in a different storage buffer formulation.
IHC staining of human liver using 16739-1-AP (same clone as 16739-1-PBS)
Immunohistochemical analysis of paraffin-embedded human liver using 16739-1-AP (TASP1 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 16739-1-PBS in a different storage buffer formulation.
IHC staining of human liver using 16739-1-AP (same clone as 16739-1-PBS)
Immunohistochemical analysis of paraffin-embedded human liver using 16739-1-AP (TASP1 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 16739-1-PBS in a different storage buffer formulation.
IHC staining of human lung using 16739-1-AP (same clone as 16739-1-PBS)
Immunohistochemical analysis of paraffin-embedded human lung using 16739-1-AP (TASP1 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 16739-1-PBS in a different storage buffer formulation.
IHC staining of human lung using 16739-1-AP (same clone as 16739-1-PBS)
Immunohistochemical analysis of paraffin-embedded human lung using 16739-1-AP (TASP1 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 16739-1-PBS in a different storage buffer formulation.
IF Staining of HeLa using 16739-1-AP (same clone as 16739-1-PBS)
Immunofluorescent analysis of HeLa cells using 16739-1-AP (TASP1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 16739-1-PBS in a different storage buffer formulation.

Featured Product
KD/KO Validated

TASP1 Polyclonal antibody, PBS Only

TASP1 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 16739-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

C20orf13, EC:3.4.25.-, Taspase 1, Taspase-1, Threonine aspartase 1

Formulation: PBS Only

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information

データシート［PDF］を印刷

MSDS

Tested Applications

Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

16739-1-PBS targets TASP1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag9918 Product name: Recombinant human TASP1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-420 aa of BC025266 Sequence: MTMEKGMSSGEGLPSRSSQVSAGKITAKELETKQSYKEKRGGFVLVHAGAGYHSESKAKEYKHVCKRACQKAIEKLQAGALATDAVTAALVELEDSPFTNAGMGSNLNLLGEIECDASIMDGKSLNFGAVGALSGIKNPVSVANRLLCEGQKGKLSAGRIPPCFLVGEGAYRWAVDHGIPSCPPNIMTTRFSLAAFKRNKRKLELAERVDTDFMQLKKRRQSSEKENDSGTLDTVGAVVVDHEGNVAAAVSSGGLALKHPGRVGQAALYGCGCWAENTGAHNPYSTAVSTSGCGEHLVRTILARECSHALQAEDAHQALLETMQNKFISSPFLASEDGVLGGVIVLRSCRCSAEPDSSQNKQTLLVEFLWSHTTESMCVGYMSAQDGKAKTHISRLPPGAVAGQSVAIEGGVCRLESPVN 相同性解析による交差性が予測される生物種
Full Name	taspase, threonine aspartase, 1
Calculated molecular weight	420 aa, 44 kDa
Observed molecular weight	50 kDa
GenBank accession number	BC025266
Gene Symbol	TASP1
Gene ID (NCBI)	55617
RRID	AB_2201218
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9H6P5
Storage Buffer	PBS only{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -80°C.

Background Information

Threonine aspartase 1 (TASP1), a particularly conserved protease that cleaves mixed lineage leukemia 1 (MLL), regulates the expression of homeotic genes and is involved in the occurrence of cancer. TASP1 drives cell cycle progression and coordinates cell proliferation and apoptosis (PMID: 33767369). Aberrant expression of TASP1 has been found in numerous liquid and solid malignancies, including leukaemia, breast cancer, lung cancer, head and neck cancer, brain cancer, colon cancer, glioblastoma and melanoma. TASP1 plays a key role in cancer cell proliferation and apoptosis and can inhibit tumor growth after knockout (PMID: 32071545, 34012990).

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of HEK-293 using 16739-1-AP (same clone as 16739-1-PBS)

WB analysis using 16739-1-AP (same clone as 16739-1-PBS)

WB analysis of HeLa using 16739-1-AP (same clone as 16739-1-PBS)

WB analysis of HeLa using 16739-1-AP (same clone as 16739-1-PBS)

IHC staining of human ovary using 16739-1-AP (same clone as 16739-1-PBS)

IHC staining of human ovary using 16739-1-AP (same clone as 16739-1-PBS)

IHC staining of human kidney using 16739-1-AP (same clone as 16739-1-PBS)

IHC staining of human kidney using 16739-1-AP (same clone as 16739-1-PBS)

IHC staining of human testis using 16739-1-AP (same clone as 16739-1-PBS)

IHC staining of human testis using 16739-1-AP (same clone as 16739-1-PBS)

IHC staining of human liver using 16739-1-AP (same clone as 16739-1-PBS)

IHC staining of human liver using 16739-1-AP (same clone as 16739-1-PBS)

IHC staining of human lung using 16739-1-AP (same clone as 16739-1-PBS)