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TDP-43 Polyclonal antibody
TDP-43 Polyclonal Antibody for FC, IF, IHC, WB, ELISA
Cat no : 10782-2-AP
Validation Data Gallery
|Positive WB detected in||SH-SY5Y cells, HeLa cells, C2C12 cells, Neuro-2a cells|
|Positive IHC detected in||human gliomas tissue, human brain (FTLD-U) tissue, mouse brain tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HeLa cells|
|Positive FC detected in||HeLa cells|
|Western Blot (WB)||WB : 1:2000-1:10000|
|Immunohistochemistry (IHC)||IHC : 1:2000-1:8000|
|Immunofluorescence (IF)||IF : 1:3000-1:12000|
|Sample-dependent, check data in validation data gallery|
10782-2-AP targets TDP-43 in WB, RIP, IP, IHC, IF, IEM, FC, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse, rat, zebrafish samples.
|Tested Reactivity||human, mouse, rat, zebrafish|
|Cited Reactivity||human, chicken, horse, Yeast, rat, seal, mouse, monkey, zebrafish, hamster|
|Host / Isotype||Rabbit / IgG|
|Immunogen||fusion protein 相同性解析による交差性が予測される生物種|
|Full Name||TAR DNA binding protein|
|Calculated molecular weight||43 kDa|
|Observed molecular weight||44 kDa|
|GenBank accession number||BC001487|
|Gene ID (NCBI)||23435|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
The TARDBP gene encodes the TDP-43 protein, initially found to repress HIV-1 transcription by binding TAR DNA. TDP-43 has since been shown to bind RNA as well as DNA, and have multiple functions in transcriptional repression, translational regulation and pre-mRNA splicing. For instance, it is reported to regulate alternate splicing of the CTFR gene. In 2006 Neumann et al. found that hyperphosphorylated, ubiquitinated and/or cleaved forms of TDP-43, collectively known as pathological TDP-43, play a major role in the disease mechanisms of ubiquitin-positive, tau- and alpha-synuclein-negative frontotemporal dementia (FTLD-U) and in amyotrophic lateral sclerosis (ALS). Proteintech's 10782-2-AP antibody is a rabbit polyclonal antibody recognizing N-terminal TDP-43. It recognizes the intact 43 kDa protein as well as all posttranslationally modified and truncated forms in multiple applications. Various forms of TDP-43 exist, including 18-35 kDa of cleaved C-terminal fragments, 45-50 kDa phospho-protein, 55 kDa glycosylated form, 75 kDa hyperphosphorylated form, and 90-300 kDa cross-linked form. (17023659, 19823856, 21666678, 22193176) Recently TDP-43 has been reported to be overexpressed in triple negative breast cancer (TNBC) and it may be a potential target for TNBC diagnosis and drug design. (29581274)
A C9orf72 promoter repeat expansion in a Flanders-Belgian cohort with disorders of the frontotemporal lobar degeneration-amyotrophic lateral sclerosis spectrum: a gene identification study.
Disruption of ER ion homeostasis maintained by an ER anion channel CLCC1 contributes to ALS-like pathologies
TDP-43 represses cryptic exon inclusion in the FTD-ALS gene UNC13A.
Neurotoxic microglia promote TDP-43 proteinopathy in progranulin deficiency.
TDP-43 loss and ALS-risk SNPs drive mis-splicing and depletion of UNC13A.
MIR-NATs repress MAPT translation and aid proteostasis in neurodegeneration.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Parijat (Verified Customer) (09-10-2023)
Good antibody for IF
Florencia (Verified Customer) (07-17-2023)
This antibody is one of the most important ones we use in the lab for studying ALS! It works very well and we will continue to use it.
Pauline (Verified Customer) (02-23-2022)
Xin (Verified Customer) (01-23-2022)
Very good in WB with a band of around 45 kD.
Jessica (Verified Customer) (06-10-2021)
good for WB and IF
Ana (Verified Customer) (03-14-2021)
TDP43 in human primary fibroblasts. 10 ug of total protein. Primary ab incubation 16h 4ºC 1:1000 in BSA 3% in PBST. Secondary ab Goat anti-rabbit HRP 1:5000 1h RT incubation.
Yasuyo (Verified Customer) (01-21-2021)
Works good for ICC in fibroblasts. High nuclear signal. Cells fixed with 4% PFA 15 min. TDP43 ab dilution 1:200 in PBST O/N 4ºC. Donkey anti-rabbit Alexafluor 568 1:500 for 1h RT.
MEIMEI (Verified Customer) (11-12-2020)
the antibody work prefect for my staining.
Stephane (Verified Customer) (10-31-2020)
Good product for staining.
Marina (Verified Customer) (10-25-2020)
TDP43 in human primary fibroblasts. Loading: 10 ug in LB with DTT. TGX 4-15% gel. Blocking: 1h 3% BSA in PBST (0.1% tween). Primary ab incubation 16h 4ºC 1:1000 in BSA 3% in PBST (0.1% Tween) Detection: Goat anti-rabbit HRP + ECL+.
Uxoa (Verified Customer) (02-19-2020)
Good ICC in human primary fibroblats. Small amount of signal in cytoplasm in UT and control cells. High nuclear signal. Fibroblats fixed with 4% PFA 15 min. TDP43 ab dilution 1:100 in PBST (0.1% triton) + 10% DS O/N 4ºC. Donkey anti-rabbit alexa fluor 555 1:500 in PBST+10%DS 1h RT.
Paul (Verified Customer) (01-15-2020)
Produces good IF images with minimal background.
Laura (Verified Customer) (01-10-2020)
Works very well
Benjamin (Verified Customer) (01-07-2020)
Works well in both western blotting and Immunofluorescence. Nice clear images obtained with IF.
Apoorva (Verified Customer) (12-18-2019)
It has great specificity over wide range of species.
Tilly (Verified Customer) (11-28-2019)
Great N-terminal TDP-43 antibody, works really well for immunohistochemistry, immunofluorescence and western blot from mouse brain tissue. Unfortunately, I have not been able to get it to work for precipitation in mouse brain.
Azita (Verified Customer) (10-04-2019)
I used it for ICC and it had low signal and high background with following (1/500, 1/300, 1/100,1/50) dilutions. Cell were fixed by 4% PFA.
Francisco (Verified Customer) (09-28-2019)
Antibody worked well. My lab has used the antibody in various experiments for quantifications, many of which were included in published works.
Alinda (Verified Customer) (09-11-2019)
Doxycycline-inducible SHSY5Y show GFP-TDP-43 expression upon dox addition.
David (Verified Customer) (07-18-2019)
Excellent for both immunoblot and immunocytochemistry. Strong signal and low background in both cases. Single band in control cells for immunoblot.
George (Verified Customer) (07-07-2019)
Good antibody to show mislocalisation in transgenic ALS disease mouse model tissue culture. Needs a high concentration to show up for microscopy but very strong and clear signal.
Elena (Verified Customer) (08-17-2018)
Petra (Verified Customer) (03-06-2018)