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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TAPBP Polyclonal antibody (30500-1-AP)

    WB analysis using 30500-1-AP (same clone as 30500-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 30500-1-AP (TAPBP antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30500-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse colon tissue using TAPBP Polyclonal antibody (30500-1-AP)

    IHC staining of mouse colon using 30500-1-AP (same clone as 30500-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 30500-1-AP (TAPBP antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30500-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse colon tissue using TAPBP Polyclonal antibody (30500-1-AP)

    IHC staining of mouse colon using 30500-1-AP (same clone as 30500-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 30500-1-AP (TAPBP antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30500-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using TAPBP Polyclonal antibody (30500-1-AP)

    IF Staining of HeLa using 30500-1-AP (same clone as 30500-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using TAPBP antibody (30500-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 30500-1-PBS in a different storage buffer formulation.

TAPBP Polyclonal antibody, PBS Only

TAPBP Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 30500-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

30500-1-PBS targets TAPBP in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag30885

Product name: Recombinant human TAPBP protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 21-120 aa of NM_003190

Sequence: GPAVIECWFVEDASGKGLAKRPGALLLRQGPGEPPPRPDLDPELYLSVHDPAGALQAAFRRYPRGAPAPHCEMSRFVPLPASAKWASGLTPAQNCPRALD

相同性解析による交差性が予測される生物種
Full Name TAP binding protein (tapasin)
Calculated molecular weight 48 kDa
Observed molecular weight48 kDa
GenBank accession numberNM_003190
Gene Symbol TAPBP
Gene ID (NCBI) 6892
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDO15533
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

TAPBP, also known as tapasin, is a V-C1 (variable-constant) immunoglobulin superfamily (IgSF) molecule. It is involved in the association of MHC class I with transporter associated with antigen processing (TAP) and in the assembly of MHC class I with peptide (PMID: 11920573).

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