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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TAP1 Polyclonal antibody (11114-1-AP)

    WB analysis using 11114-1-AP (same clone as 11114-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 11114-1-AP (TAP1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11114-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using TAP1 Polyclonal antibody (11114-1-AP)

    WB analysis of HeLa using 11114-1-AP (same clone as 11114-1-PBS)

    WB result of TAP1 antibody (11114-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TAP1 transfected HeLa cells. This data was developed using the same antibody clone with 11114-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using TAP1 Polyclonal antibody (11114-1-AP)

    WB analysis using 11114-1-AP (same clone as 11114-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 11114-1-AP (TAP1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11114-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse skeletal muscle tissue using TAP1 Polyclonal antibody (11114-1-AP)

    WB analysis of mouse skeletal muscle using 11114-1-AP (same clone as 11114-1-PBS)

    mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 11114-1-AP (TAP1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11114-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HepG2 cells using TAP1 Polyclonal antibody (11114-1-AP)

    IP experiment of HepG2 using 11114-1-AP (same clone as 11114-1-PBS)

    IP result of anti-TAP1 (IP:11114-1-AP, 4ug; Detection:11114-1-AP 1:3000) with HepG2 cells lysate 1320 ug. This data was developed using the same antibody clone with 11114-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using TAP1 Polyclonal antibody (11114-1-AP)

    IHC staining of human pancreas cancer using 11114-1-AP (same clone as 11114-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 11114-1-AP (TAP1 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 11114-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using TAP1 Polyclonal antibody (11114-1-AP)

    IHC staining of human pancreas cancer using 11114-1-AP (same clone as 11114-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 11114-1-AP (TAP1 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 11114-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using TAP1 Polyclonal antibody (11114-1-AP)

    IF Staining of HepG2 using 11114-1-AP (same clone as 11114-1-PBS)

    Immunofluorescent analysis of HepG2 cells, using TAP1 antibody 11114-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye). This data was developed using the same antibody clone with 11114-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using TAP1 Polyclonal antibody (11114-1-AP)

    IF Staining of HepG2 using 11114-1-AP (same clone as 11114-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 11114-1-AP (TAP1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 11114-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

TAP1 Polyclonal antibody, PBS Only

TAP1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 11114-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

ABCB2, ATP-binding cassette sub-family B member 2, EC:7.4.2.14, PSF1, PSF-1

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

11114-1-PBS targets TAP1 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag1619

Product name: Recombinant human TAP1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 509-808 aa of BC014081

Sequence: LYQMQFTQAVEVLLSIYPRVQKAVGSSEKIFEYLDRTPRCPPSGLLTPLHLEGLVQFQDVSFAYPNRPDVLVLQGLTFTLRPGEVTALVGPNGSGKSTVAALLQNLYQPTGGQLLLDGKPLPQYEHRYLHRQVAAVGQEPQVFGRSLQENIAYGLTQKPTMEEITAAAVKSGAHSFISGLPQGYDTEVDEAGSQLSGGQRQAVALARALIRKPCVLILDDATSALDANSQLQVEQLLYESPERYSRSVLLITQHLSLVEQADHILFLEGGAIREGGTHQQLMEKKGCYWAMVQAPADAPE

相同性解析による交差性が予測される生物種
Full Name transporter 1, ATP-binding cassette, sub-family B (MDR/TAP)
Calculated molecular weight 81 kDa
Observed molecular weight 70-81 kDa
GenBank accession numberBC014081
Gene Symbol TAP1
Gene ID (NCBI) 6890
RRIDAB_2200201
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ03518
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

TAP1, also known as ABCB2, PSF1 or RING4, is a member of the ATP-binding cassette (ABC) family of transmembrane transporters and is an essential component of the major histocompatibility complex (MHC) class I antigen-presenting pathway. TAP is involved in the transport of antigens from the cytoplasm to the endoplasmic reticulum for association with MHC class I molecules. It also acts as a molecular scaffold for the final stage of MHC class I folding. Defects in TAP1 are a cause of bare lymphocyte syndrome type 1 (BLS1). Western blot analysis using this antibody detected a major band around 70-80 kDa in HeLa cells.

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