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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TAOK3 Monoclonal antibody (67451-1-Ig)

    WB analysis using 67451-1-Ig (same clone as 67451-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67451-1-Ig (TAOK3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67451-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using TAOK3 Monoclonal antibody (67451-1-Ig)

    WB analysis of HeLa using 67451-1-Ig (same clone as 67451-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 67451-1-Ig (TAOK3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67451-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using TAOK3 Monoclonal antibody (67451-1-Ig)

    WB analysis using 67451-1-Ig (same clone as 67451-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67451-1-Ig (TAOK3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67451-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Jurkat cells using TAOK3 Monoclonal antibody (67451-1-Ig)

    WB analysis of Jurkat using 67451-1-Ig (same clone as 67451-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 67451-1-Ig (TAOK3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67451-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using TAOK3 Monoclonal antibody (67451-1-Ig)

    WB analysis of HepG2 using 67451-1-Ig (same clone as 67451-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 67451-1-Ig (TAOK3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67451-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using TAOK3 Monoclonal antibody (67451-1-Ig)

    IHC staining of human prostate cancer using 67451-1-Ig (same clone as 67451-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 67451-1-Ig (TAOK3 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67451-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using TAOK3 Monoclonal antibody (67451-1-Ig)

    IHC staining of human prostate cancer using 67451-1-Ig (same clone as 67451-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 67451-1-Ig (TAOK3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67451-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using TAOK3 Monoclonal antibody (67451-1-Ig)

    IHC staining of mouse kidney using 67451-1-Ig (same clone as 67451-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 67451-1-Ig (TAOK3 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67451-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human prostate cancer tissue using TAOK3 Monoclonal antibody (67451-1-Ig)

    IF Staining of human prostate cancer using 67451-1-Ig (same clone as 67451-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human prostate cancer tissue using TAOK3 antibody (67451-1-Ig, Clone: 1A5A9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67451-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human prostate cancer tissue using TAOK3 Monoclonal antibody (67451-1-Ig)

    IF Staining of human prostate cancer using 67451-1-Ig (same clone as 67451-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human prostate cancer tissue using TAOK3 antibody (67451-1-Ig, Clone: 1A5A9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67451-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using TAOK3 Monoclonal antibody (67451-1-Ig)

    IF Staining of HeLa using 67451-1-Ig (same clone as 67451-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using TAOK3 antibody (67451-1-Ig, Clone: 1A5A9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 67451-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

TAOK3 Monoclonal antibody, PBS Only

TAOK3 Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA
Cat No. 67451-1-PBS
Clone No.1A5A9

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IF-P, ELISA

Synonyms

JIK, 1A5A9, CTCL-associated antigen HD-CL-09, Cutaneous T-cell lymphoma-associated antigen HD-CL-09, Dendritic cell-derived protein kinase

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67451-1-PBS targets TAOK3 in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag29146

Product name: Recombinant human TAOK3 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 366-430 aa of BC002756

Sequence: SMQEVMDESSSELVMMHDDESTINSSSSVVHKKDHVFIRDEAGHGDPRPEPRPTQSVQSQALHYR

相同性解析による交差性が予測される生物種
Full Name TAO kinase 3
Calculated molecular weight 105 kDa
Observed molecular weight 100-105 kDa
GenBank accession numberBC002756
Gene Symbol TAOK3
Gene ID (NCBI) 51347
RRIDAB_2882685
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDQ9H2K8
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

TAOK3(Serine/threonine-protein kinase TAO3) is also named as DPK, JIK, KDS, MAP3K18 and belongs to the STE Ser/Thr protein kinase family. It acts as an activator of the p38/MAPK14 stress-activated MAPK cascade. In response to DNA damage, it is involved in the G2/M transition DNA damage checkpoint by activating the p38/MAPK14 stress-activated MAPK cascade, probably by mediating phosphorylation of upstream MAP2K3 and MAP2K6 kinases. And this protein can be autophosphorylated(PMID:20949042).

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