Validation Data Gallery

Filter: AllWBIP

WB analysis of HeLa using 10544-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 10544-1-AP (TAF9 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of MCF-7 using 10544-1-AP
MCF-7 cells were subjected to SDS PAGE followed by western blot with 10544-1-AP (TAF9 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of human colon using 10544-1-AP
human colon tissue were subjected to SDS PAGE followed by western blot with 10544-1-AP (TAF9 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of human skeletal muscle using 10544-1-AP
human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 10544-1-AP (TAF9 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of rat liver using 10544-1-AP
rat liver tissue were subjected to SDS PAGE followed by western blot with 10544-1-AP (TAF9 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 10544-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 10544-1-AP (TAF9 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of MCF-7 using 10544-1-AP
MCF7 cells were subjected to SDS PAGE followed by western blot with 10544-1-AP (TAF9 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of human heart using 10544-1-AP
human heart tissue were subjected to SDS PAGE followed by western blot with 10544-1-AP (TAF9 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 10544-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 10544-1-AP (TAF9 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IP experiment of mouse skeletal muscle using 10544-1-AP
IP result of anti-TAF9 (IP:10544-1-AP, 4ug; Detection:10544-1-AP 1:1000) with mouse skeletal muscle tissue lysate 2200ug.

Featured Product
KD/KO Validated

TAF9 Polyclonal antibody

TAF9 Polyclonal Antibody for WB, IP, ELISA

Cat No. 10544-1-AP

Publications(7)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, ELISA

Synonyms

AD 004, AK6, CGI 137, CINAP, CIP, STAF31/32, TAF2G, TAF9, TAFII 31, TAFII 32, TAFII31, TAFII32, TAFIID32

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (7)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HeLa cells, MCF-7 cells, human colon tissue, human skeletal muscle tissue, HEK-293 cells, human heart tissue, rat liver tissue
Positive IP detected in	mouse skeletal muscle tissue

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 6 publications below
IHC	See 2 publications below
IP	See 1 publications below

Product Information

10544-1-AP targets TAF9 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag0833 Product name: Recombinant human TAF9 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-172 aa of BC007426 Sequence: MLLPNILLTGTPGVGKTTLGKELASKSGLKYINVGDLAREEQLYDGYDEEYDCPILDEDRVVDELDNQMREGGVIVDYHGCDFFPERWFHIVFVLRTDTNVLYERLETRGYNEKKLTDNIQCEIFQVLYEEATASYKEEIVHQLPSNKPEELENNVDQILKWIEQWIKDHNS 相同性解析による交差性が予測される生物種
Full Name	TAF9 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 32kDa
Calculated molecular weight	28 kDa
Observed molecular weight	24-28 kDa
GenBank accession number	BC007426
Gene Symbol	TAF9
Gene ID (NCBI)	6880
RRID	AB_2271416
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q16594
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

TAF9, also named as TAF2G, TAFII31, is a 264 amino acid protein, which belongs to the TAF9 family. TAF9 are involved in transcriptional activation as well as repression of distinct but overlapping sets of genes. TAF9 may have a role in gene regulation associated with apoptosis.

Protocols

Product Specific Protocols
IP protocol for TAF9 antibody 10544-1-AP	Download protocol
WB protocol for TAF9 antibody 10544-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	IHC	Cancers (Basel) Identification of Prognostic Markers of Gynecologic Cancers Utilizing Patient-Derived Xenograft Mouse Models. Authors - Ha-Yeon Shin View Article
human	WB	Front Oncol Stable Isotope Tracing Uncovers Reduced γ/β-ATP Turnover and Metabolic Flux Through Mitochondrial-Linked Phosphotransfer Circuits in Aggressive Breast Cancer Cells. Authors - Aleksandr Klepinin View Article
Human	WB	J Biol Chem The Cell Surface Hyaluronidase TMEM2 Regulates Cell Adhesion and Migration via Degradation of Hyaluronan at Focal Adhesion Sites. Authors - Fumitoshi Irie View Article
mouse,rat	WB,IHC,IP	Front Pharmacol Activation of TAF9 via Danshensu-Induced Upregulation of HDAC1 Expression Alleviates Non-alcoholic Fatty Liver Disease. Authors - Ruiwen Wang KD Validated View Article
human	WB	J Proteome Res Targeted Proteomic Analysis Revealed Kinome Reprogramming during Acquisition of Radioresistance in Breast Cancer Cells. Authors - Weili Miao View Article
human	WB	Mol Cell Proteomics Multi-Level Proteomics Reveals Epigenetic Signatures in BCG-mediated Macrophage Activation Authors - Zoe Schaefer View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of HeLa using 10544-1-AP

WB analysis of MCF-7 using 10544-1-AP

WB analysis of human colon using 10544-1-AP

WB analysis of human skeletal muscle using 10544-1-AP

WB analysis of rat liver using 10544-1-AP

WB analysis of HeLa using 10544-1-AP

WB analysis of MCF-7 using 10544-1-AP

WB analysis of human heart using 10544-1-AP

WB analysis of HEK-293 using 10544-1-AP

IP experiment of mouse skeletal muscle using 10544-1-AP

TAF9 Polyclonal antibody

TAF9 Polyclonal Antibody for WB, IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Identification of Prognostic Markers of Gynecologic Cancers Utilizing Patient-Derived Xenograft Mouse Models.

Stable Isotope Tracing Uncovers Reduced γ/β-ATP Turnover and Metabolic Flux Through Mitochondrial-Linked Phosphotransfer Circuits in Aggressive Breast Cancer Cells.

The Cell Surface Hyaluronidase TMEM2 Regulates Cell Adhesion and Migration via Degradation of Hyaluronan at Focal Adhesion Sites.

Activation of TAF9 via Danshensu-Induced Upregulation of HDAC1 Expression Alleviates Non-alcoholic Fatty Liver Disease.

Targeted Proteomic Analysis Revealed Kinome Reprogramming during Acquisition of Radioresistance in Breast Cancer Cells.

Multi-Level Proteomics Reveals Epigenetic Signatures in BCG-mediated Macrophage Activation