Validation Data Gallery
Tested Applications
| Positive WB detected in | HeLa cells, mouse testis tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
32590-1-AP targets TAF7L in WB, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag38542 Product name: Recombinant human TAF7L protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 361-462 aa of NM_024885.3 Sequence: DCSEEYLERQLQAEFIESGQYRANEGTSSIVMEIQKQIEKKEKKLHKIQNKAQRQKDLIMKVENLTLKNHFQSVLEQLELQEKQKNEKLISLQEQLQRFLKK* 相同性解析による交差性が予測される生物種 |
| Full Name | TAF7-like RNA polymerase II, TATA box binding protein (TBP)-associated factor, 50kDa |
| Calculated molecular weight | 53kDa，462aa |
| Observed molecular weight | 53 kDa |
| GenBank accession number | NM_024885.3 |
| Gene Symbol | TAF7L |
| Gene ID (NCBI) | 54457 |
| RRID | AB_3742665 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity Purification |
| UNIPROT ID | Q5H9L4 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
TAF7L encodes a transcription initiation factor that functions as a TAF7-like component of the TFIID general transcription factor complex. It is similar to a mouse gene encoding a TATA box binding protein-associated factor, and it shows testis-specific expression. The protein is associated with RNA polymerase II preinitiation complex assembly, transcription initiation at RNA polymerase II promoters, cell differentiation, and spermatogenesis.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for TAF7L antibody 32590-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |