Validation Data Gallery
Tested Applications
| Positive WB detected in | HEK-293 cells, HeLa cells, HepG2 cells, L02 cells, LNCaP cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
33029-1-AP targets TAF4 in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag38833 Product name: Recombinant human TAF4 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 450-590 aa of NM_003185.4 Sequence: QLPPGMVLVRSENGQLLMIPQQALAQMQAQAHAQPQTTMAPRPATPTSAPPVQISTVQAPGTPIIARQVTPTTIIKQVSQAQTTVQPSATLQRSPGVQPQLVLGGAAQTASLGTATAVQTGTPQRTVPGATTTSSAATETM 相同性解析による交差性が予測される生物種 |
| Full Name | TAF4 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 135kDa |
| Calculated molecular weight | 110 kDa |
| Observed molecular weight | 110 kDa |
| GenBank accession number | NM_003185.4 |
| Gene Symbol | TAF4 |
| Gene ID (NCBI) | 6874 |
| RRID | AB_3742826 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity Purification |
| UNIPROT ID | O00268 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
TAF4, also named as TAF2C, TAF2C1, TAF4A, TAFII130 and TAFII135, belongs to the TAF4 family. TAF4 makes part of TFIID is a multimeric protein complex that plays a central role in mediating promoter responses to various activators and repressors. TAF4 potentiates transcriptional activation by the AF-2S of the retinoic acid, vitamin D3 and thyroid hormone.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for TAF4 antibody 33029-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |