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Species Reactivity
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Conjugate
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Conjugate
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

TAF10 Polyclonal antibody

TAF10 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 20346-1-AP

Synonyms

STAF28, TAF(II)30, TAF10, TAF2A, TAF2H, TAFII 30, TAFII30

Validation Data Gallery

Applications:
  • WB analysis of mouse testis using 20346-1-AP

    WB analysis of mouse testis using 20346-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 20346-1-AP (TAF10 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse testis using 20346-1-AP

    IHC staining of mouse testis using 20346-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 20346-1-AP (TAF10 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse testis using 20346-1-AP

    IHC staining of mouse testis using 20346-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 20346-1-AP (TAF10 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inmouse testis tissue, rat testis tissue
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:500
Immunohistochemistry (IHC)IHC : 1:200-1:800
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 20346-1-AP is TAF10 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TAF10 fusion protein Ag14153 相同性解析による交差性が予測される生物種
Full Name TAF10 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 30kDa
Calculated molecular weight 218 aa, 22 kDa
Observed molecular weight 29 kDa
GenBank accession numberBC012088
Gene symbol TAF10
Gene ID (NCBI) 6881
RRIDAB_2878678
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for TAF10 antibody 20346-1-APDownload protocol
IHC protocol for TAF10 antibody 20346-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols