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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

TACSTD2/TROP2 Polyclonal antibody

TACSTD2/TROP2 Polyclonal Antibody for WB, IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Cat no : 29856-1-AP

Synonyms

EGP 1, GA733, GA733 1, M1S1, TACSTD2, TROP2

Validation Data Gallery

Applications:
  • WB analysis using 29856-1-AP

    WB analysis using 29856-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29856-1-AP (TACSTD2/TROP2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of HaCaT using 29856-1-AP

    IF Staining of HaCaT using 29856-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed HaCaT cells using TACSTD2/TROP2 antibody (29856-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inA431 cells, HaCaT cells
Positive IF detected inHaCaT cells
Planning an IHC experiment? We recommend our IHCeasy TACSTD2 Ready-To-Use IHC Kit. TACSTD2 primary antibody included.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)IF : 1:200-1:800
Sample-dependent, check data in validation data gallery

Product Information

29856-1-AP targets TACSTD2/TROP2 in WB, IF, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TACSTD2/TROP2 fusion protein Ag31473 相同性解析による交差性が予測される生物種
Full Name tumor-associated calcium signal transducer 2
Calculated molecular weight 36 kDa
Observed molecular weight45-50 kDa
GenBank accession numberBC009409
Gene symbol TACSTD2
Gene ID (NCBI) 4070
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Trophoblast cell surface antigen 2 (TROP2), also named as TACSTD2, is a transmembrane glycoprotein. TROP2 plays a role in transducing intracellular calcium signals. It is expressed in trophoblast cells, cornea and multi-stratified epithelia. TROP2 is overexpressed in most carcinomas and is involved in cancer proliferation, migration, invasion, and metastasis. Congenital mutations of TROP2 cause a rare autosomal recessive disease which may lead to blindness-the gelatinous drop-like corneal disease (PMID: 35688908).

Protocols

Product Specific Protocols
WB protocol for TACSTD2/TROP2 antibody 29856-1-APDownload protocol
IF protocol for TACSTD2/TROP2 antibody 29856-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols