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Validation Data Gallery

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  • Western Blot (WB) analysis of HaCaT cells using TACSTD2/TROP2 Polyclonal antibody (27360-1-AP)

    WB analysis of HaCaT using 27360-1-AP (same clone as 27360-1-PBS)

    HaCaT cells were subjected to SDS PAGE followed by western blot with 27360-1-AP (TACSTD2 antibody) at dilution of 1:1200 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27360-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using TACSTD2/TROP2 Polyclonal antibody (27360-1-AP)

    IHC staining of human breast cancer using 27360-1-AP (same clone as 27360-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27360-1-AP (TACSTD2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27360-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using TACSTD2/TROP2 Polyclonal antibody (27360-1-AP)

    IHC staining of human breast cancer using 27360-1-AP (same clone as 27360-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27360-1-AP (TACSTD2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27360-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using TACSTD2/TROP2 Polyclonal antibody (27360-1-AP)

    IHC staining of human prostate cancer using 27360-1-AP (same clone as 27360-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 27360-1-AP (TACSTD2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27360-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using TACSTD2/TROP2 Polyclonal antibody (27360-1-AP)

    IHC staining of human prostate cancer using 27360-1-AP (same clone as 27360-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 27360-1-AP (TACSTD2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27360-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HaCaT cells using TACSTD2/TROP2 Polyclonal antibody (27360-1-AP)

    IF Staining of HaCaT using 27360-1-AP (same clone as 27360-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HaCaT cells using TACSTD2/TROP2 antibody (27360-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 27360-1-PBS in a different storage buffer formulation.

TACSTD2/TROP2 Polyclonal antibody, PBS Only

TACSTD2/TROP2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 27360-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

TACSTD2, M1S1, GA733-1, GA733 1, GA733

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

27360-1-PBS targets TACSTD2/TROP2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag25769

Product name: Recombinant human TACSTD2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 138-215 aa of BC009409

Sequence: KGDLSLRCDELVRTHHILIDLRHRPTAGAFNHSDLDAELRRLFRERYRLHPKFVAAVHYEQPTIQIELRQNTSQKAAG

相同性解析による交差性が予測される生物種
Full Name tumor-associated calcium signal transducer 2
Calculated molecular weight 36 kDa
Observed molecular weight45-55 kDa
GenBank accession numberBC009409
Gene Symbol TROP2
Gene ID (NCBI) 4070
RRIDAB_2918122
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP09758
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Trophoblast cell surface antigen 2 (TROP2), also named as TACSTD2, is a transmembrane glycoprotein. TROP2 plays a role in transducing intracellular calcium signals. It is expressed in trophoblast cells, cornea and multi-stratified epithelia. TROP2 is overexpressed in most carcinomas and is involved in cancer proliferation, migration, invasion, and metastasis. Congenital mutations of TROP2 cause a rare autosomal recessive disease which may lead to blindness-the gelatinous drop-like corneal disease (PMID: 35688908).

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