Validation Data Gallery
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Tested Applications
| Positive WB detected in | HeLa cells, PC-3 cells |
| Positive FC (Intra) detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 1 publications below |
| IHC | See 1 publications below |
Product Information
25270-1-AP targets TACR2 in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide 相同性解析による交差性が予測される生物種 |
| Full Name | tachykinin receptor 2 |
| Calculated molecular weight | 44 kDa |
| Observed molecular weight | 44 kDa |
| GenBank accession number | NM_001057 |
| Gene Symbol | TACR2 |
| Gene ID (NCBI) | 6865 |
| RRID | AB_2879998 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P21452 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
The tachykinins represent a family of peptide neurotransmitters including substance P, neurokinin A and neurokinin B, which act through the stimulation of tachykinin NK1, NK2 or NK3 receptors. NK2, also known as TACR2 (tachykinin receptor 2), is a receptor for the tachykinin neurokinin A (neuropeptide substance K). It is associated with G proteins that activate a phosphatidylinositol-calcium second messenger system.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for TACR2 antibody 25270-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cancer Cell Int TACR2 is associated with the immune microenvironment and inhibits migration and proliferation via the Wnt/β-catenin signaling pathway in prostate cancer. | ||
Mol Med Rep Occurrence of substance P and neurokinin receptors during the early phase of spinal fusion. |